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| Status |
Public on Dec 31, 2013 |
| Title |
Geriatric muscle stem cells switch reversible quiescence into senescence (Set 2; Old/Geriatric vs. Young and SAMR1 vs. SAMP8) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Regeneration of skeletal muscle depends on a population of adult stem cells (satellite cells) that remain quiescent throughout life. Satellite cell regenerative functions decline with aging and in progeric conditions. Here we report that geriatric satellite cells, compared to old cells, are incapable of maintaining their normal quiescent state in muscle homeostatic conditions, and this irreversibly affects their intrinsic regenerative and self-renewal capacities.
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| Overall design |
We analyzed the global changes in gene expression occurring within muscle stem cells (satellite cells) in homeostatic conditions during physiological aging and progeria. Pure satellite cell populations from dissociated skeletal muscle from Young (2-3 months), Old (22-24 months), Geriatric (28-30 months), SAMR1 and SAMP8 mice were isolated using a well-established flow cytometry protocol gating on integrin a7(+)/CD34(+) (positive selection) and Lin- (CD31, CD45, CD11b, Sca1) (negative selection).
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| Contributor(s) |
Perdiguero E, Munoz-Canoves P |
| Citation(s) |
24522534 |
| Submission date |
Dec 30, 2013 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Eusebio Perdiguero |
| E-mail(s) |
perdiguero@gmail.com
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| Organization name |
UPF
|
| Department |
Department of Medicine and Life Sciences
|
| Lab |
Cell Biology
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (14)
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| GSM1299438 |
Satellite_cells_Young_mice_biological_replicate_1 [Set 2] |
| GSM1299439 |
Satellite_cells_Young_mice_biological_replicate_2 [Set 2] |
| GSM1299440 |
Satellite_cells_Young_mice_biological_replicate_3 [Set 2] |
| GSM1299441 |
Satellite_cells_Old_mice_biological_replicate_1 [Set 2] |
| GSM1299442 |
Satellite_cells_Old_mice_biological_replicate_2 [Set 2] |
| GSM1299443 |
Satellite_cells_Old_mice_biological_replicate_3 [Set 2] |
| GSM1299444 |
Satellite_cells_Geriatric_mice_biological_replicate_1 [Set 2] |
| GSM1299445 |
Satellite_cells_Geriatric_mice_biological_replicate_3 [Set 2] |
| GSM1299446 |
Satellite_cells_SAMR1_mice_biological_replicate_1 [Set 2] |
| GSM1299447 |
Satellite_cells_SAMR1_mice_biological_replicate_2 [Set 2] |
| GSM1299448 |
Satellite_cells_SAMR1_mice_biological_replicate_3 [Set 2] |
| GSM1299449 |
Satellite_cells_SAMP8_mice_biological_replicate_1 [Set 2] |
| GSM1299450 |
Satellite_cells_SAMP8_mice_biological_replicate_2 [Set 2] |
| GSM1299451 |
Satellite_cells_SAMP8_mice_biological_replicate_3 [Set 2] |
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| This SubSeries is part of SuperSeries: |
| GSE53728 |
Geriatric muscle stem cells switch reversible quiescence into senescence |
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| Relations |
| BioProject |
PRJNA232728 |
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