|
| Status |
Public on Jan 31, 2014 |
| Title |
Identification of biomarkers for Miltefosine resistance in Leishmania donovani |
| Organism |
Leishmania donovani |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify biomarkers specific for Miltefosin resistant parasites, we used DNA microarray to identify differentially expressed genes in resistant parasite in comparision to sensitive and further validated with real time PCR assay and immunoblotting assay
|
| |
|
| Overall design |
Clinically proven miltefosine reistant as well as respondant parasite strains isolated from splenic aspirates and grown in M199 media. For microarray resistant and sensitive samples run in duplicate while for real time PCR, 25 resistant and 33 sensitive strains used. For immunoblotting, confirmed genes were cloned and recombinant protein blotted against miltefosine failure and treated patients sera.
|
| |
|
| Contributor(s) |
Tiwary P, Kumar D, Rai M, Sundar S |
| Citation(s) |
29280431 |
| Submission date |
Dec 31, 2013 |
| Last update date |
May 22, 2019 |
| Contact name |
PUJA TIWARY |
| E-mail(s) |
tiwarypuja@gmail.com
|
| Phone |
919452071217
|
| Organization name |
Banaras Hindu University
|
| Department |
MEDICINE
|
| Lab |
INFECTIOUS DISEASE RESEARCH LABORATORY
|
| Street address |
C/O Prof. Shyam Sundar, Dept of Medicine
|
| City |
Varanasi |
| State/province |
India |
| ZIP/Postal code |
221005 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL18125 |
Leishmania donovani Gene Expression 8x15k Microarray - Genotypic Technology Private Limited (AMADID: 035638) (condensed) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA232750 |
Less...
More...