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Series GSE5381 Query DataSets for GSE5381
Status Public on Mar 01, 2007
Title Gene expression analysis of liver and kidney following methapyrilene treatment in male Sprague-Dawley rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary This study was performed to gain insight into the hepatotoxicity of methapyrilene. Gene expression changes in the kidney (non-target tissue for toxicity) were compared to those in the liver (target tissue for toxicity) to attribute gene expression changes to relevant biological processes (i.e. toxic response, non-specific acute response, pharmacological response, etc…).
Keywords: Time course, tissue comparison, liver toxicity, hepatotoxicity
 
Overall design Male Sprague-Dawley rats were treated with methapyrilene at two different doses (10 mg/kg/day and 100 mg/kg/day) for 1, 3, and 7 days. Livers and kidneys were removed 24 hours after the last dose and flash frozen in liquid nitrogen. Five hundred nanograms (500 ng) of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer’s protocol. For each two color comparison, 750 ng each of Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v. 7.1) using defaults for all parameters.

Equal amounts of RNA was pooled from all of the animals in each group (n=4). Each treated group was hybridized against its pooled time-matched and tissue-matched control group in quadruplicate (2 replicates + 2 fluor reversals).
 
Contributor(s) Auman JT, Chou J, Gerrish K, Huang Q, Jayadev S, Blanchard K
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Submission date Jul 24, 2006
Last update date Dec 06, 2012
Contact name James Todd Auman
E-mail(s) jtauman@email.unc.edu
Phone (919) 966-9942
Organization name University of North Carolina at Chapel Hill
Department Institute for Pharmacogenomics and Individualized Therapy
Street address 3304 Kerr Hall, Campus Box 7360
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7360
Country USA
 
Platforms (1)
GPL890 Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version)
Samples (48)
GSM122894 MP G3 high 1 day vs. MP G1 control 1 day (ExpID 1994)
GSM122895 MP G5 low 3 days vs. MP G4 control 3 days (ExpID 1992)
GSM122896 MP G6 high 3 days vs. MP G4 control 3 days (ExpID 1995)
Relations
BioProject PRJNA96287

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5381_RAW.tar 1.4 Gb (http)(custom) TAR (of TIFF, TXT)

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