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Series GSE53810 Query DataSets for GSE53810
Status Public on Jan 14, 2014
Title Switching of the Relative Dominance Between Feedback Mechanisms in Lipopolysaccharide-Induced NF-kB Signaling
Organism Mus musculus
Experiment type Expression profiling by array
Summary The bacterial product lipopolysaccharide (LPS) stimulates nuclear factor kB (NF-kB) signaling, which results in the production of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), as part of the immune response. NF-kB target genes also include those encoding proteins that inhibit NF-kB signaling through negative feedback loops. By simultaneously studying the dynamics of the nuclear translocation of the NF-kB subunit RelA and the activity of a Tnf-driven reporter in a mouse macrophage cell line, Sung et al. found that the gene encoding RelA was also a target of NF-kB. Synthesis of RelA occurred only at higher concentrations of LPS and constituted a positive feedback loop that dominated over existing negative feedback mechanisms. Genes expressed in response to a high concentration of LPS were enriched for those involved in innate immune responses. Together, these data suggest that the RelA-dependent positive feedback loop enables macrophages to mount an effective immune only above a critical concentration of LPS.
Overall design Bone-marrow-derived macrophage (BMDM) cells were stimulated with zero, low, and high concentration of LPS separately for 4hrs. Two replicates for each condition.
Contributor(s) Sung MH, Li N, Lao Q, Gottschalk R, Hager G, Fraser I
Citation(s) 24425788
Submission date Jan 03, 2014
Last update date Jun 14, 2018
Contact name Timothy G Myers
Organization name National Institute of Allergy and Infectious Diseases
Department Research Technologies Branch
Lab Genomic Technologies Section
Street address 50 South Drive, Room 5509
City Bethesda
State/province MD
ZIP/Postal code 20892-8005
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1301135 BMDM_10ng_4hr_2nd
GSM1301136 BMDM_Zero_4hr_1st
GSM1301137 BMDM_0.1ng_4hr_1st
BioProject PRJNA233402

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53810_RAW.tar 6.1 Mb (http)(custom) TAR (of TXT)
GSE53810_non-normalized.txt.gz 1020.9 Kb (ftp)(http) TXT
Raw data are available on Series record
Raw data provided as supplementary file
Processed data included within Sample table

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