|
| Status |
Public on Mar 05, 2015 |
| Title |
Mapping of genome-wide DSBs in HEK293T cells using the RAFT procedure. |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Large DNA fragments (mainly of 50-250 kb, denoted as forum domains) were isolated inside agarose plugs as described below in the extract protocol section. After ligation of 5'-bio-oligos to the blunt DNA termini the digestion with Sau3A enzyme was performed followed by selection of genome-wide preparation of forum domains termini on streptavidin-paramegnetic particles. After ligation of Sau3A adapter the termini were PCR amplified and used for deep sequencing.
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| |
|
| Overall design |
Mapping of Double-Strand DNA Breaks/Forum Termini
|
| |
|
| Contributor(s) |
Tchurikov NA, Kravatsky YV |
| Citation(s) |
25627242, 26484232 |
| Submission date |
Jan 03, 2014 |
| Last update date |
Jun 07, 2019 |
| Contact name |
Nickolai Tchurikov |
| E-mail(s) |
tchurikov@eimb.ru
|
| Organization name |
Engelhardt Institute of Molecular Biology
|
| Department |
Department of Epigenetic Mechanisms of Gene Expression Regulation
|
| Street address |
32, Vavilova str.
|
| City |
Moscow |
| ZIP/Postal code |
119991 |
| Country |
Russia |
| |
|
| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA233403 |
| SRA |
SRP034918 |
