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| Status |
Public on Jun 01, 2007 |
| Title |
Knock-down of three core transcription factors in hEC cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Three transcription factors were silenced in human embryonic carcinoma cells, OCT4, NANOG, and SOX2. Downstream effects were analysed by means of microarrays to obtain insights into the regulation of self-renewal in these cells.
Keywords: RNAi-chip
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| |
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| Overall design |
Cells were transfected with esiRNA against the respective transcription factor transcripts. Knock-down efficiencies were high as monitored by real-time RT-PCR and Western blotting. Samples for chip hybridisations were harvested 2.5 days after transfection.
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| |
|
| Contributor(s) |
Greber B, Lehrach H, Adjaye J |
| Citation(s) |
17506876 |
| Submission date |
Aug 01, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL2507 |
Sentrix Human-6 Expression BeadChip |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA96079 |
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