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Status |
Public on Jan 25, 2014 |
Title |
Over-expression of either MECP2_e1 or MECP2_e2 in mouse MECP2 knockout fibroblasts |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Aim: to detect genes that are differentially transcribed in mouse MECP2 knockout fibroblasts over-expressing either of the two human MECP2 isoforms, MECP2_e1 or MECP2_e2. Methods: mouse Mecp2tm1.1Bird knockout fibroblast cell line was stably infected by lentiviral vectors over-expressing MECP2_e1, MECP2_e2, or eGFP. RNA extracted, and used for gene expression microarray analysis
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Overall design |
3 biological replicates for MECP2_e1; 3 replicates for MECP2_e2; 3 replicates for eGFP as control; 3 replicates for MECP2_e1 with 9bp insertion mutation in N-terminal
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Contributor(s) |
Vincent JB, Orlic-Milacic M |
Citation(s) |
24699272 |
Submission date |
Jan 21, 2014 |
Last update date |
Jan 12, 2017 |
Contact name |
john vincent |
E-mail(s) |
john.vincent@camh.ca
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Organization name |
Centre for Addiction & Mental Health
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Department |
Neuroscience Research
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Lab |
Molecular Neuropsychiatry & Development (MiND) Lab
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Street address |
250 College Street
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City |
Toronto |
State/province |
ON |
ZIP/Postal code |
M5T 1R8 |
Country |
Canada |
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Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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Samples (12)
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Relations |
BioProject |
PRJNA236055 |