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Series GSE54261 Query DataSets for GSE54261
Status Public on Jan 25, 2014
Title Over-expression of either MECP2_e1 or MECP2_e2 in mouse MECP2 knockout fibroblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary Aim: to detect genes that are differentially transcribed in mouse MECP2 knockout fibroblasts over-expressing either of the two human MECP2 isoforms, MECP2_e1 or MECP2_e2.
Methods: mouse Mecp2tm1.1Bird knockout fibroblast cell line was stably infected by lentiviral vectors over-expressing MECP2_e1, MECP2_e2, or eGFP. RNA extracted, and used for gene expression microarray analysis
 
Overall design 3 biological replicates for MECP2_e1; 3 replicates for MECP2_e2; 3 replicates for eGFP as control; 3 replicates for MECP2_e1 with 9bp insertion mutation in N-terminal
 
Contributor(s) Vincent JB, Orlic-Milacic M
Citation(s) 24699272
Submission date Jan 21, 2014
Last update date Jan 12, 2017
Contact name john vincent
E-mail(s) john.vincent@camh.ca
Organization name Centre for Addiction & Mental Health
Department Neuroscience Research
Lab Molecular Neuropsychiatry & Development (MiND) Lab
Street address 250 College Street
City Toronto
State/province ON
ZIP/Postal code M5T 1R8
Country Canada
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1311244 mouse ko fibroblast eGFP1
GSM1311245 mouse ko fibroblast eGFP2
GSM1311246 mouse ko fibroblast eGFP3
Relations
BioProject PRJNA236055

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54261_RAW.tar 190.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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