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| Status |
Public on Aug 04, 2006 |
| Title |
Expression profiling of A.thaliana with the posttranscriptionally silenced Myosin XI-K |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
This study was aimed to determine roles of individual myosins in organelle trafficking in plant cell. Expression of Myosin XI-K was suppressed in transgenic A.thaliana by using RNAi approach. To ensure that the RNAi-induced gene silencing was specific and did not affect expression of other myosins, a microarray analysis was performed.
Keywords: RNAi-mediated posttranscriptional gene silencing, Myosin expression levels
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| Overall design |
Total RNA was purified from A.thaliana plants transformed with an inverted repeat construct harbouring a sequence derived from Myosin XI-K ORF. 3 independent groups of plants were used for comparison of the expression profiles of 17 myosin RNAs to a control group comprised of plants constitutively expressing GUS ORF as a transgene.
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| Contributor(s) |
Peremyslov V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 01, 2006 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Valera Peremyslov |
| E-mail(s) |
peremisv@bcc.orst.edu
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| Phone |
541-737-5481
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| Fax |
541-737-3573
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| Organization name |
Oregon State University
|
| Department |
Botany and Plant Pathology
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| Street address |
2082 Cordley Hall
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| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97331 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (4)
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| GSM124351 |
A.thaliana expressing GUS transgene |
| GSM124453 |
A.thaliana with the RNAi silenced Myosin XI-K, Group 1 |
| GSM124460 |
A.thaliana with the RNAi silenced Myosin XI-K, Group 2 |
| GSM124461 |
A.thaliana with the RNAi silenced Myosin XI-K, Group 3 |
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| Relations |
| BioProject |
PRJNA96093 |
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