|
| Status |
Public on Jan 23, 2014 |
| Title |
shRNA Screening Identifies JMJD1C as being required for Leukemia Maintenance (Agilent) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We used a mouse model of human AML induced by the MLL-AF9 fusion oncogene, and an epigenetic shRNA library to screen for novel potential drug targets. One of the best candidate drug targets identified in these screens was Jmjd1c. Depletion of Jmjd1c impairs growth and colony formation of mouse MLL-AF9 cells in vitro, as well as establishment of leukemia after transplantation. Depletion of JMJD1C impairs expansion and colony formation of human leukemic cell lines, with the strongest effect observed in the MLL-rearranged ALL cell line, SEM. In both mouse and human leukemic cells, the growth defect upon JMJD1C depletion appears to be primarily due to increased apoptosis, which implicates JMJD1C as a potential therapeutic target in leukemia.
|
| |
|
| Overall design |
To assess the effect of JMJD1C depletion on transcription, we compared the transcriptome of shJMJD1C- and shScr-transduced mouse MLL-AF9 transformed cells 48h after infection. A total of 451 transcripts were detected as changing between the two conditions (FDR<0.25)
|
| |
|
| Contributor(s) |
Helin K |
| Citation(s) |
24501218 |
| Submission date |
Jan 22, 2014 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Kristian Helin |
| E-mail(s) |
kristian.helin@bric.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
BRIC
|
| Street address |
Ole Maaløes vej 5
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA236136 |
Less...
More...