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Series GSE54313 Query DataSets for GSE54313
Status Public on Mar 19, 2014
Title Expression analysis of Mll3 knockdown p53-/- HSPC vs. control p53-/- HSPC
Organism Mus musculus
Experiment type Expression profiling by array
Summary Recurring deletions of chromosome 7 and 7q [-7/del(7q)] occur in myelodysplastic syndromes and acute myeloid leukemia (AML) and are associated with poor prognosis. However, the identity of specific tumor suppressors on 7q remains elusive. Using RNAi and CRISPR/Cas9 approaches, we show that a ~50% reduction in gene dosage of the mixed lineage leukemia 3 (MLL3) gene, located on 7q36.1, cooperates with other events occurring in -7/del(7q) AMLs to promote leukemogenesis. Mll3 suppression impairs the differentiation of HSPC. Interestingly, Mll3 suppressed leukemias, like human -7/del(7q) AMLs, are refractory to conventional chemotherapy but sensitive to the BET inhibitor JQ1. Thus, our mouse model functionally validates MLL3 as a haploinsufficient 7q tumor suppressor, and suggests a therapeutic option for this aggressive disease.
 
Overall design Total RNA obtained from sorted lin-ckit+ hematopoietic stem and progenitor cells of recipient mice transplanted with shRen;p53-/- or shMll3;p53-/- cells at 6 weeks after transplant
 
Contributor(s) Chen C, Liu Y, Lowe SW
Citation(s) 24794707
Submission date Jan 22, 2014
Last update date Jun 14, 2018
Contact name Chong Chen
E-mail(s) chen_chong@yahoo.com
Organization name Sichuan University
Department State Key Laboratory of Biotherapy
Lab Chong Chen
Street address 17 Renmin S. Road-No. 3
City Chengdu
State/province Sichuan
ZIP/Postal code 610041
Country China
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1312822 shRen;p53-/- HSCPC Rep1
GSM1312823 shRen;p53-/- HSCPC Rep2
GSM1312824 shRen;p53-/- HSCPC Rep3
Relations
BioProject PRJNA236139

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54313_RAW.tar 3.1 Mb (http)(custom) TAR
GSE54313_non_normalized.txt.gz 2.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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