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| Status |
Public on Mar 01, 2014 |
| Title |
Heart tissue from Nelf-b CreER mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Negative elongation factor (NELF) is known to enforce promoter-proximal pausing of RNA polymerase II (Pol II), a pervasive phenomenon observed across multicellular genomes. However, the physiological impact of NELF on tissue homeostasis remains unclear. Here we show for the first time that whole-body conditional deletion of the B subunit of NELF (NELF-B) in adult mice results in cardiomyopathy and impaired response to cardiac stress. Tissue-specific knockout of NELF-B confirms its cell-autonomous function in cardiomyocytes. NELF directly supports transcription of those genes encoding rate-limiting enzymes in fatty acid oxidation and the tricarboxylic acid (TCA) cycle. NELF also shares extensively transcriptional target genes with peroxisome proliferator-activated receptors alpha (PPARalpha), a master regulator of energy metabolism in myocardium. Mechanistically, NELF helps stablize the transcription initation complex at the metabolism-related genes. Our findings strongly indicate that NELF is part of the PPARalpha-mediated transcription regulatory network that maintains metabolic homeostasis in cardiomyocytes.
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| Overall design |
3 Nelf-b f/f and 3 Nelf-b f/f; CreER female mice were injected with Tamoxifen at 8 wk old. Heart tissue were harvested at 20 wks old and used for RNA preparation.
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| Contributor(s) |
Pan H, Guo Z, April C, Fan J, Wang D, Li R |
| Citation(s) |
24656816 |
| Submission date |
Jan 24, 2014 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Haihui Pan |
| E-mail(s) |
panh@livemail.uthscsa.edu
|
| Phone |
210-562-4159
|
| Organization name |
University of Texas Health Science Center at San Antonio
|
| Department |
Molecular Medicine
|
| Lab |
Dr. Rong Li
|
| Street address |
8403 Floyd Curl Dr.
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA236353 |
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