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Series GSE54372 Query DataSets for GSE54372
Status Public on Mar 01, 2014
Title Heart tissue from Nelf-b CreER mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Negative elongation factor (NELF) is known to enforce promoter-proximal pausing of RNA polymerase II (Pol II), a pervasive phenomenon observed across multicellular genomes. However, the physiological impact of NELF on tissue homeostasis remains unclear. Here we show for the first time that whole-body conditional deletion of the B subunit of NELF (NELF-B) in adult mice results in cardiomyopathy and impaired response to cardiac stress. Tissue-specific knockout of NELF-B confirms its cell-autonomous function in cardiomyocytes. NELF directly supports transcription of those genes encoding rate-limiting enzymes in fatty acid oxidation and the tricarboxylic acid (TCA) cycle. NELF also shares extensively transcriptional target genes with peroxisome proliferator-activated receptors alpha (PPARalpha), a master regulator of energy metabolism in myocardium. Mechanistically, NELF helps stablize the transcription initation complex at the metabolism-related genes. Our findings strongly indicate that NELF is part of the PPARalpha-mediated transcription regulatory network that maintains metabolic homeostasis in cardiomyocytes.
 
Overall design 3 Nelf-b f/f and 3 Nelf-b f/f; CreER female mice were injected with Tamoxifen at 8 wk old. Heart tissue were harvested at 20 wks old and used for RNA preparation.
 
Contributor(s) Pan H, Guo Z, April C, Fan J, Wang D, Li R
Citation(s) 24656816
Submission date Jan 24, 2014
Last update date Jun 14, 2018
Contact name Haihui Pan
E-mail(s) panh@livemail.uthscsa.edu
Phone 210-562-4159
Organization name University of Texas Health Science Center at San Antonio
Department Molecular Medicine
Lab Dr. Rong Li
Street address 8403 Floyd Curl Dr.
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1314041 Heart_Ctrl_Rep1
GSM1314042 Heart_Ctrl_Rep2
GSM1314043 Heart_Ctrl_Rep3
Relations
BioProject PRJNA236353

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54372_RAW.tar 3.1 Mb (http)(custom) TAR
GSE54372_non_normalized.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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