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Series GSE54481 Query DataSets for GSE54481
Status Public on Feb 14, 2014
Title Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The nuclear phase of the gene expression pathway culminates in the export of mature mRNAs to the cytoplasm through nuclear pore complexes (NPCs). GANP (Germinal-centre Associated Nuclear Protein) promotes the transfer to NPCs of mRNAs bound to the transport factor NXF1. Here, we demonstrate that GANP, subunit of the TREX-2 mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression.
 
Overall design We used gene expression profiling to compare the abundance of cytoplasmic RNAs after GANP or NXF1 depletion
 
Contributor(s) Wickramasinghe V, Laskey R
Citation(s) 24510098
Submission date Jan 28, 2014
Last update date Feb 18, 2019
Contact name Vihandha Wickramasinghe
Organization name MRC Cancer Unit, Hutchison/MRC Research Centre
Lab Venkitaraman
Street address Hills Road
City Cambridge
ZIP/Postal code CB2 0XZ
Country United Kingdom
 
Platforms (1)
GPL6884 Illumina HumanWG-6 v3.0 expression beadchip
Samples (9)
GSM1316554 Control Cytoplasmic RNA Experiment 1
GSM1316555 GANP KD Cytoplasmic RNA Experiment 1
GSM1316556 NXF1 KD Cytoplasmic RNA Experiment 1
Relations
BioProject PRJNA236606

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54481_RAW.tar 6.3 Mb (http)(custom) TAR
GSE54481_non-normalized.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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