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Series GSE54569 Query DataSets for GSE54569
Status Public on Jan 31, 2014
Title The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The neural fate commitment of pluripotent stem cells requires repression of extrinsic inhibitory signals and activation of intrinsic positive transcription factors. However, it remains elusive how these two events are integrated to ensure appropriate neural conversion. Here, we show that Oct6 functions as an essential positive factor for neural differentiation of mouse embryonic stem cells (ESCs), specifically during the transition from epiblast stem cells (EpiSCs) to neural progenitor cells (NPCs). Chimera analysis showed that Oct6 knockdown leads to markedly decreased incorporation of ESC in neuroectoderm. By contrast, Oct6-overexpressing ESC derivatives preferentially contribute to neuroectoderm. Genome-wide ChIP-seq and RNA-seq analyses indicate that Oct6 is an upstream activator of neural lineage genes, and also a repressor of BMP and Wnt signalings. Our results establish Oct6 as a critical regulator that promotes neural commitment of pluripotent stem cells through a dual role: activating internal neural induction programs and antagonizing extrinsic neural inhibitory signals.
 
Overall design RNA-seq was performed to examine Oct6 function in ESC neural differentiation at Day2, Day4 and Day6 after dox induction. On Day4 EB, ChIP-seq assay was ultilized to characterize the targets of Oct6.
 
Contributor(s) Zhu Q, Song L, Peng G, Sun N
Citation(s) 24929964, 26484290
Submission date Jan 30, 2014
Last update date May 15, 2019
Contact name Guangdun Peng
E-mail(s) guangdun.peng@gmail.com
Organization name Chinese Academy of Sciences
Department Guangzhou Institutes of Biomedicine and Health
Lab Stem cell and early development
Street address 190 Kaiyuan Road
City Guangzhou
ZIP/Postal code 510530
Country China
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1319108 fbs_D2
GSM1319109 fbs_D4
GSM1319110 fbs_D6
This SubSeries is part of SuperSeries:
GSE69865 Role of Pou3f1 during mouse pluripotent stem cell neural fate commitment
Relations
BioProject PRJNA237042
SRA SRP036080

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54569_Oct6OE.genes.fpkm.txt.gz 488.6 Kb (ftp)(http) TXT
GSE54569_mESC_EB_D4_Oct6.peaks.bed.gz 95.9 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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