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Series GSE54594 Query DataSets for GSE54594
Status Public on Jan 31, 2015
Title Dose-specific transcriptional responses in thyroid tissue in mice after 131I administration
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptomic profiling of normal mouse thyroid tissue following 131At irradiation
In the present investigation, microarray analysis was used to monitor transcriptional activity in thyroids in mice 24 h after 131I exposure. The aims of this study were to 1) assess the transcriptional patterns associated with 131I exposure in normal mouse thyroid tissue and 2) identify novel biomarkers for 131I exposure.
Methods:
Adult BALB/c nude mice were i.v. injected with 13, 130 or 260 kBq of 131I and killed 24h after injection. The corresponding absorbed doses delivered to the thyroid gland were 0.85, 8.5, or 17 Gy. Mock-treated mice were used as controls. Total RNA was extracted from thyroids and processed using MouseRef-8 Whole-Genome Expression BeadChips (Illumina). Nexus Expression 2.0 software were used for analysis.
Results:
In total, 497, 546, and 90 transcripts were regulated in the thyroid after 0.85, 8.5, and 17 Gy, respectively. These were involved in several biological functions, e.g. oxygen access, inflammation and immune response, and apoptosis/anti-apoptosis. Approximately 50% of the involved transcripts at each absorbed dose level were dose-specific and 18 transcripts were commonly detected at all absorbed dose levels. The Agpat9, Plau, Prf1, and S100a8 gene expression displayed a monotone decrease in regulation with absorbed dose, and may function as dose-related biomarkers for 131I exposure.
Conclusion:
Distinct and substantial differences in gene expression and affected biological functions were detected at the different absorbed dose levels. The transcriptional profiles were specific for the different absorbed dose levels. We propose that the Agpat9, Plau, Prf1, and S100a8 genes are novel potential absorbed dose-related biomarkers for 131I exposure.
 
Overall design Total RNA was isolated from fresh-frozen tissue samples (Normal Balb/c mouse thyroids)
 
Contributor(s) Rudqvist N, Schüler E, Parris TZ, Langen B, Helou K, Forssell-Aronsson E
Citation(s) 25496975, 26492889
Submission date Jan 31, 2014
Last update date Jan 14, 2021
Contact name Nils Rudqvist
E-mail(s) nils.rudqvist@radfys.gu.se
Organization name Gothenburg university
Street address Gula stråket 2b
City Göteborg
State/province VG
ZIP/Postal code 41345
Country Sweden
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM1319640 131I_thyroid_A
GSM1319641 131I_thyroid_B
GSM1319642 131I_thyroid_C
Relations
BioProject PRJNA237106

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54594_RAW.tar 3.1 Mb (http)(custom) TAR
GSE54594_non-normalized.txt.gz 327.2 Kb (ftp)(http) TXT
GSE54594_normalized.txt.gz 381.8 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record
Processed data are available on Series record

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