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| Status |
Public on Feb 04, 2014 |
| Title |
TGF-β/Smad3 Stimulates Stem Cell/Developmental Gene Expression and Vascular Smooth Muscle Cell De-Differentiation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to determine a mechanism through which TGF-b/Smad3 promote these effects, Affymetrix gene expression arrays were performed on primary rat SMCs infected with Smad3 and stimulated with TGF-b or infected with GFP alone.
More than 200 genes were differentially expressed (>2.0 fold change, p<0.05) in TGF-b/Smad3 stimulated SMCs. We then performed GO term enrichment analysis using the DAVID bioinformatics database and found that TGF-b/Smad3 activated the expression of multiple genes related to either development or cell differentiation, several of which have been shown to be associated with multipotent stem or progenitor cells.
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| Overall design |
Vascular smooth muscle cells from 3 rats were infected with adenovirus expressing Smad3 and treated with TGF-B for 24 h (TGF-B/Smad3 group). Adenovirus expressing GFP (GFP group) was control. TGF-B/Smad3 treated or control cells were used for RNA extraction and hybridization on Affymetrix microarrays (n=3).
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| Contributor(s) |
Shi X, DiRenzo D, Guo L, Franco SR, Wang B, Seedial S, Kent KC |
| Citation(s) |
24718260 |
| Submission date |
Feb 03, 2014 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Xudong daniel Shi |
| E-mail(s) |
daniel3678@yahoo.com
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| Phone |
608-213-6358
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| Organization name |
University of Wisconsin
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| Department |
Surgery
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| Street address |
1111 Highland Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA237211 |
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