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Series GSE54681 Query DataSets for GSE54681
Status Public on Nov 03, 2014
Title Genome-wide changes in gene expression during the development and after recovery from heart failure in a mouse model of transient cardiomyopathy.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Altered myocardial gene expression from heart failure (HF) has mostly been identified by single-point analysis of end-stage disease. This may miss earlier changes in gene expression that are transient and/or directionally opposite to those observed later. By sampling left ventricular myocardial tissue at different time points in a mouse model of cardiomyopathy, we examined differentially expressed transcripts between non-failing controls, early-HF (2 and 3 days after cardiac insult), peak-HF (10 days) and after recovery from HF (28 days). As a results, we found that gene expression followed varying patterns: Downregulation of metabolic pathways occurred early and was sustained into late stage-HF. In contrast, most signaling pathways undergo a complex biphasic pattern: Calcium signaling, p53, apoptosis and MAP-kinase pathways displayed a bi-directional response, declining early but rising late, while NF-κB-related transcription displayed the opposite pattern. The HF-induced transcriptomic changes were reversed after recovery at 28 days. We conclude that the myocardial transcriptome in evolving HF displays distinct and dynamic transcript clusters. Therapies targeting different phases of HF should consider these dynamic features when identifying high-value targets.
 
Overall design Mice expressing mutated estrogen receptor-coupled Cre under transcriptional control of cardiac-specific myosin heavy chain (MerCreMer, MCM, 005650;Jackson Laboratories, Bar Harbor, ME) were back-crossed for >10 generations with C57Bl6/J. Transient cardiomyopathy develops in these mice following 5 day exposure to tamoxifen free base (T5648, Sigma), with peak dysfunction at day 10 and full recovery after 4 weeks. Full transcriptome analyses were generated from flash-frozen left ventricular myocardium at different time points (control = baseline, day 2, day 3, day 10, and day 28).
 
Contributor(s) Barth AS, Rowell J, Kass DA
Citation(s) 25159852
Submission date Feb 05, 2014
Last update date Jun 14, 2018
Contact name Andreas Sebastian Barth
E-mail(s) abarth3@jhmi.edu
Phone +1 410 614 0032
Organization name Johns Hopkins University
Department Medicine, Division of Cardiology
Lab Tomaselli
Street address 720 Rutland Ave.
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (19)
GSM1322060 control 1
GSM1322061 control 2
GSM1322062 control 3
Relations
BioProject PRJNA237353

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54681_RAW.tar 3.1 Mb (http)(custom) TAR
GSE54681_non-normalized.txt.gz 2.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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