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Series GSE54723 Query DataSets for GSE54723
Status Public on Dec 05, 2014
Title Nutritional Strategy to Prevent Fatty Liver and Insulin Resistance Independent of Obesity by Reducing GIP Responses
Organism Mus musculus
Experiment type Expression profiling by array
Summary High intakes of carbohydrates, particularly sucrose, in Western societies are associated with the development of non-alcoholic fatty liver (NAFL) and diabetes mellitus. It is unclear whether this is related to the carbohydrate quantity or the hormonal responses, particularly Glucose-dependent Insulinotropic Polypeptide (GIP) which is released in the proximal intestine. We, therefore, used the glucose-fructose dimer as proximally resorbed, 1,4-linked sucrose or distally resorbed 1,6-linked palatinose. Palatinose compared to sucrose, indeed, resulted in slower glucose absorption and reduced postprandial insulin and GIP levels. After 22 weeks of isocaloric diets, palatinose feeding prevented hepatic steatosis (48.5%) compared to sucrose and improved glucose tolerance, without differences in body composition and food intake.
Ablation of GIP receptor (GIPR) signaling in Gipr-/- mice completely prevented the deleterious metabolic effects of sucrose feeding. Furthermore, our microarray analysis indicated that sucrose increased the hepatic expression of Suppressor of Cytokine Signaling 2 (SOCS2), which is involved in the growth hormone signaling pathway and participates in the development of NAFL. Our results suggest that the site of glucose absorption and the GIP response determine liver fat accumulation and insulin resistance. GIP may play a role in sucrose induced fatty liver by regulating the expression of SOCS2.
 
Overall design Expression data from liver tissue of mice fed with isocaloric diets containing either sucrose or palatinose
 
Contributor(s) Keyhani-Nejad F, Birkenfeld AL, Irmler M, Beckers J, Pfeiffer AH
Citation(s) 25348610
Submission date Feb 06, 2014
Last update date Jun 14, 2018
Contact name Johannes Beckers
E-mail(s) beckers@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (14)
GSM1322809 Liver tissue from mice fed with a sucrose-containing diet for 22 weeks, biological replicate 1
GSM1322810 Liver tissue from mice fed with a sucrose-containing diet for 22 weeks, biological replicate 2
GSM1322811 Liver tissue from mice fed with a sucrose-containing diet for 22 weeks, biological replicate 3
Relations
BioProject PRJNA237500

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54723_RAW.tar 3.1 Mb (http)(custom) TAR
GSE54723_non-normalized.txt.gz 950.2 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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