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Series GSE54820 Query DataSets for GSE54820
Status Public on Feb 10, 2014
Title Strigolactone analogs induce apoptosis through activation of p38 and the stress response pathway in cancer cell lines and in conditionally reprogrammed primary prostate cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Strigolactones are a novel class of plant hormones produced in roots and regulate shoot and root development. We have previously shown that synthetic strigolactone analogues potently inhibit growth of breast cancer cells and breast cancer stem cells. Here we show that strigolactone analogues inhibit the growth and survival of an array of cancer-derived cell lines representing solid and non-solid cancer cells including: prostate, colon, lung, melanoma, osteosarcoma and leukemic cell lines, while normal cells were minimally affected. Furthermore, we tested the response of patient-matched conditionally reprogrammed normal and prostate cancer cells. The tumor cells exhibited significantly higher sensitivity to the two most potent SL analogues with increased apoptosis compared to their normal counterpart cells. Treatment of cancer cells with strigolactone analogues was hallmarked by increased expression and activity of genes involved in stress signaling, cell cycle arrest and apoptosis. All five strigolactone analogues induced G2/M cell cycle arrest, accompanied with a decrease in the expression level of cyclin B1. Apoptosis was marked by increased percentages of cells in the sub-G1 fraction and was confirmed by Annexin V staining. In conditionally reprogramed matched tumor and normal prostate cells, the cleavage of PARP1 confirmed the specific increase in apoptosis of tumor cells. In summary, Strigolactone analogues are promising candidates for anticancer therapy by their ability to specifically induce cell cycle arrest, cellular stress and apoptosis in tumor cells with minimal effects on growth and survival of normal cells.
 
Overall design There are duplicate samples for each condition. U2OS cells were treated with 2 different strigolactone analogues: ST362 or MEB55 at the concentration of 5 ppm for either 6 hr or 24 hr.Control samples were those treated with vehicle only .
 
Contributor(s) Pollock CB, McDoungh S, Wang VS, Lee HC, Ringer L, Lee RJ, Feldman AS, Prandi C, Koltai H, Kapulnik Y, Rudrigaz O, Schlegel R, Albanese C, Yarden RI
Citation(s) 24742967
Submission date Feb 10, 2014
Last update date Aug 13, 2018
Contact name Ronit Iris Yarden
E-mail(s) riy2@georgetown.edu
Phone 202-6876872
Organization name Georgetown University
Department Human Science
Street address 3900 Reservoir Rd., NW
City Washington
State/province DC
ZIP/Postal code 20007
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (12)
GSM1324450 Control 6hr_rep1
GSM1324451 Control 6hr_rep2
GSM1324452 ST-362 6hr_rep1
Relations
BioProject PRJNA237754

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54820_RAW.tar 26.2 Mb (http)(custom) TAR
GSE54820_non-normalized_data.txt.gz 2.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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