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Series GSE5483 Query DataSets for GSE5483
Status Public on Jul 31, 2008
Title Transcriptional Profiling of Responses to Estrogen and Progesterone in Mammary Gland
Organism Mus musculus
Experiment type Expression profiling by array
Summary Epidemiological studies have shown that a full-term pregnancy at early age can decrease the breast cancer risk up to one-half. Pregnancy has been shown to prevent carcinogen-induced mammary tumors in rodents as well. The protective effect of pregnancy can be mimicked by administration of estrogen and progesterone to nulliparous rodents in amounts that are similar to those during pregnancy and alters responsiveness of p53 to DNA damage.
Ovariectomized mice were treated with estrogen (E), progesterone (P), both estrogen and progesterone (E + P), or vehicle (V) alone and global expression profiles was analyzed to identify the mechanisms by which estrogen and progesterone combine to sensitize p53 function.
Keywords: comparative hormone treatment
 
Overall design Twenty ovariectomized animals were used for examine transcriptional effects of hormones by microarray. The hormones were administered by daily i.p. injection for 4 days.Treatment groups included: 4 animals receiving 2ug 17-beta-estrogen (E), 4 animals receiving 1mg progesterone (P), 5 animals receiving both estrogen and progesterone (E+P) and 4 animals receiving 100ul sesame oil (V). Epithelial-free fat pads from 3 animals receiving E+P were also analyzed to distinguish responses to E+P in the stroma (E+P CFP). Lymph nodes were removed from mammary glands at the time of collection.
 
Contributor(s) Lu S, Becker KA, Minter LM, Roberts AL, Mathews L, Schneider SS, Loureiro O, Guo A, Siegelmann HT, MacBeth KJ, Sebastiani P, Tirrell SM, Jerry DJ
Citation(s) 18556351
Submission date Aug 09, 2006
Last update date Feb 18, 2018
Contact name D. Joseph Jerry
E-mail(s) jjerry@vasci.umass.edu
Phone 413-545-5335
Fax 413-545-6326
URL http://htpp://www.umass.edu/vasci/faculty/jerry/jerry.html
Organization name University of Massachusetts
Department Veterinary & Animal Scineces
Street address 161 Holdsworth Way
City Amherst
State/province MA
ZIP/Postal code 01003
Country USA
 
Platforms (3)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
GPL83 [MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array
Samples (60)
GSM126397 mammary gland-2ug estrogen intraperitoneally-biologic replicate 1
GSM126398 mammary gland-2ug estrogen intraperitoneally-biologic replicate 2
GSM126399 mammary gland-2ug estrogen intraperitoneally-biologic replicate 3
Relations
BioProject PRJNA96001

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