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Series GSE54839 Query DataSets for GSE54839
Status Public on Apr 01, 2014
Title A molecular profile of chronic cocaine abuse includes differential expression of genes regulating transcription, chromatin and dopamine cell phenotype
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Midbrain dopamine (DA)-synthesizing neurons play a key role in the addiction process, providing a compelling rationale for determining drug-induced molecular changes arising in these cells. This microarray-based study determined the profiles of midbrain gene expression in chronic cocaine abusers (n = 10) and well-matched drug-free control subjects (n = 10). Array-related procedures were performed in triplicate for each subject. Data analysis revealed that 98 array probes (corresponding to 91 genes) exhibited robust, statistically significant expression differences between chronic cocaine abusers and matched control subjects (p ≤ 0.05, FDR = 5%; with ≥ 1.4 fold-change cut-off applied). Changes in transcript abundance identified by microarray were validated by qPCR analysis in every instance examined (n = 11), regardless of the direction or magnitude of change, supporting the validity of the larger dataset of genes differentially expressed in cocaine abusers. Many of the genes exhibiting robust differential expression were associated with the regulation of transcription, chromatin function, or dopamine cell phenotype. For approximately one-half of these genes, transcript abundance was significantly predictive for subject assignment to the cocaine-abusing versus control cohort. The findings suggest that there is a molecular signature associated with core pathophysiological changes in the DA neurons of chronic cocaine abusers that can be exploited for the development of potential biomarkers and novel therapeutic targets for addiction.
Overall design Human post-mortem midbrain gene expression derived from cocaine-related deaths are compared to midbrain gene expression of non-cocaine related deaths. Each subject was was arrayed in triplicates.
Contributor(s) Bannon MJ, Michelhaugh SK, Hartley ZJ, Halter SD, David JA, Schmidt CJ, Johnson MM
Citation(s) 24642598
Submission date Feb 10, 2014
Last update date Aug 16, 2018
Contact name Michael J Bannon
Phone (313) 993-4271
Organization name Wayne State University School of Medicine
Department Pharmacology
Lab 3302 Scott Hall
Street address 540 E Canfield
City Detroit
State/province MI
ZIP/Postal code 48202
Country USA
Platforms (1)
GPL6947 Illumina HumanHT-12 V3.0 expression beadchip
Samples (60)
GSM1324893 con-7_A
GSM1324894 con-7_B
GSM1324895 con-7_C
BioProject PRJNA239360

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54839_RAW.tar 80.0 Mb (http)(custom) TAR (of IDAT)
GSE54839_non-normalized.txt.gz 14.4 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

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