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| Status |
Public on Jun 25, 2014 |
| Title |
VDR primary targets by genome-wide transcriptional profiling |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We carried out a genome-wide investigation of the primary transcriptional targets of 1α,25(OH)2D3 in breast epithelial cancer cells using RNA-Seq technology. We identified early transcriptional targets of 1α,25(OH)2D3 involved in adhesion, growth regulation, angiogenesis, actin cytoskeleton regulation, hexose transport, inflammation and immunomodulation, apoptosis, endocytosis and signaling. Furthermore, we found several transcription factors to be regulated by 1α,25(OH)2D3 that subsequently amplify and diversify the transcriptional output driven by 1α,25(OH)2D3 leading finally to a growth arrest of the cells. Moreover, we could show that 1α,25(OH)2D3 elevates the trimethylation of histone H3 lysine 4 at several target gene promoters. Our present transcriptomic analysis of differential expression after 1α,25(OH)2D3 treatment provides a resource of primary 1α,25(OH)2D3 targets that might drive the antiproliferative action in breast cancer epithelial cells.
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| Overall design |
ChIP-Seq for trimethylated histone H3K4 with SKBr3 cells treated for 2h with 100nM 1α,25(OH)2D3 or vehicle as control.
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| Contributor(s) |
Goeman F, De Nicola F, D’Onorio De Meo P, Elmi B, Pallocca M, Castrignanò T, Pesole G, Strano S, Blandino G, Fanciulli M, Muti P |
| Citation(s) |
24726990 |
| Submission date |
Feb 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Matteo Pallocca |
| Organization name |
Italian National Cancer Institute "Regina Elena"
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| Street address |
Via Fermo Ognibene
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| City |
Rome |
| ZIP/Postal code |
00144 |
| Country |
Italy |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA237824 |
| SRA |
SRP037554 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54856_RAW.tar |
483.4 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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