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Series GSE5497 Query DataSets for GSE5497
Status Public on Aug 12, 2006
Title Pyruvate induces mitochondrial biogenesis by a PGC-1alpha independent mechanism
Organism Mus musculus
Experiment type Expression profiling by array
Summary The present study examines the impact of altering energy provision on mitochondrial biogenesis in muscle cells. C2C12 myoblasts were chronically treated with supraphysiological levels of sodium pyruvate for 72 hr. Treated cells exhibited increased mitochondrial protein expression, basal respiratory rate and maximal oxidative capacity. The increase in mitochondrial biogenesis was independent of increases in PGC-1alpha and PGC-1alpha mRNA expression. To further assess whether PGC-1alpha expression was necessary for pyruvate action, cells were infected with adenovirus containing shRNA for PGC-1alpha prior to treatment with pyruvate. Despite a 70% reduction in PGC-1alpha mRNA the effect of pyruvate was preserved. Furthermore, pyruvate induced mitochondrial biogenesis in primary myoblasts from PGC-1alpha null mice. These data suggest that regulation of mitochondrial biogenesis by pyruvate in myoblasts is independent of PGC-1alpha, suggesting the existence of a novel energy-sensing pathway regulating oxidative capacity.

Keywords: basal state versus treatment at one time point
Overall design C2C12 myoblasts were incubated with either basal media or basal media supplemented with 50 mM sodium pyruvate for 72 hr. Total RNA was extracted using TRIzol and GeneChip experiment was conducted using Gene Chip Mouse Genome 430 2.0 Expression Array. A Gene Chip analysis for each condition was performed in triplicate.
Contributor(s) Wilson L, Yang Q, Gullicksen PS, Halse R
Citation(s) 17182725
Submission date Aug 10, 2006
Last update date Feb 11, 2019
Contact name Reza Halse
Organization name Novartis Institutes for BioMedical Research
Street address 100 Technology Square
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM126586 myoblast_untreated_rep1
GSM126587 myoblast_untreated_rep2
GSM126588 myoblast_untreated_rep3
BioProject PRJNA96139

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