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Status |
Public on Jun 02, 2014 |
Title |
Expression data from RCAS PDGFB/Nestin tv-A glioma cells within Ptprd+/+p16-/- (n=2), Ptprd+/-p16-/- (n=2), and Ptrpd-/-p16-/- (n=3) mice. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
PTPRD is a tumor suppressor of glioma that is frequently co-deleted with CDKN2A/p16. We show that Ptprd and p16 cooperate to promote gliomagenesis in the RCAS PDGFB / Nestin tv-A glioma mouse model. We found unique gene expression changes within tumor cells of Ptprd+/-p16-/- vs. Ptprd+/+p16-/- and Ptprd-/-p16-/- tumor cells.
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Overall design |
Neonatal mice were injected with RCAS PDGFB GFP. At symptoms, or at 12 weeks post injection, GFP+DAPI- tumor cells were sorted for RNA extraction and hybridization on Affymetrix microarrays.
The mouse strain used was a mixed background of C57/BL6 and FVB/N. Ptprd mice were from Uetani et al. 2000 and p16/Nestin-tvA mice were from Tchouganouva et al. 2007.
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Contributor(s) |
Ortiz B, Chan TA |
Citation(s) |
24843164 |
Submission date |
Feb 14, 2014 |
Last update date |
May 04, 2018 |
Contact name |
Timothy Chan |
E-mail(s) |
chant@mskcc.org
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Organization name |
Memorial Sloan-Kettering Cancer Center
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Department |
Human Oncology and Pathogenesis Program
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Street address |
1275 York Avenue
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
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Platforms (1) |
GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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Samples (7)
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Relations |
BioProject |
PRJNA238357 |