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Series GSE55061 Query DataSets for GSE55061
Status Public on Jun 02, 2014
Title Expression data from RCAS PDGFB/Nestin tv-A glioma cells within Ptprd+/+p16-/- (n=2), Ptprd+/-p16-/- (n=2), and Ptrpd-/-p16-/- (n=3) mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary PTPRD is a tumor suppressor of glioma that is frequently co-deleted with CDKN2A/p16. We show that Ptprd and p16 cooperate to promote gliomagenesis in the RCAS PDGFB / Nestin tv-A glioma mouse model.
We found unique gene expression changes within tumor cells of Ptprd+/-p16-/- vs. Ptprd+/+p16-/- and Ptprd-/-p16-/- tumor cells.
 
Overall design Neonatal mice were injected with RCAS PDGFB GFP. At symptoms, or at 12 weeks post injection, GFP+DAPI- tumor cells were sorted for RNA extraction and hybridization on Affymetrix microarrays.

The mouse strain used was a mixed background of C57/BL6 and FVB/N. Ptprd mice were from Uetani et al. 2000 and p16/Nestin-tvA mice were from Tchouganouva et al. 2007.
 
Contributor(s) Ortiz B, Chan TA
Citation(s) 24843164
Submission date Feb 14, 2014
Last update date May 04, 2018
Contact name Timothy Chan
E-mail(s) chant@mskcc.org
Organization name Memorial Sloan-Kettering Cancer Center
Department Human Oncology and Pathogenesis Program
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (7)
GSM1328942 Ptprd+/+p16-/- tumor, biological rep 1
GSM1328943 Ptprd+/+p16-/- tumor, biological rep 2
GSM1328944 Ptprd+/-p16-/- tumor, biological rep 1
Relations
BioProject PRJNA238357

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Supplementary file Size Download File type/resource
GSE55061_RAW.tar 13.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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