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| Status |
Public on Feb 14, 2015 |
| Title |
Expression data from isolated E17.5 mouse skin with or without Ovol2 overexpression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
During epithelial tissue morphogenesis, developmental progenitor cells undergo dynamic adhesive and cytoskeletal remodeling to trigger proliferation and migration. Transcriptional mechanisms that restrict such mild form of epithelial plasticity to maintain lineage-restricted differentiation in committed epithelial tissues are poorly understood. Here we report that simultaneous ablation of transcriptional repressor-encoding Ovol1 and Ovol2 results in expansion and blocked terminal differentiation of embryonic epidermal progenitor cells. Conversely, mice overexpressing Ovol2 in their skin epithelia exhibit precocious differentiation accompanied by smaller progenitor cell compartments. We show that Ovol1/2-deficient epidermal cells fail to undertake alpha-catenin–driven actin cytoskeletal reorganization and adhesive maturation, and exhibit changes that resemble epithelial-to-mesenchymal transition (EMT). Remarkably, these alterations as well as defective terminal differentiation are reversed upon depletion of EMT-promoting transcriptional factor Zeb1. Collectively, our findings reveal Ovol-Zeb1-a-catenin sequential repression and highlight novel functions of Ovol as gatekeepers of epithelial adhesion and differentiation by inhibiting progenitor-like traits and epithelial plasticity.
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| Overall design |
Skin from control and Ovol2 overexpression (Ovol2 BT) were physically isolated for RNA extraction and hybridization on Affymetrix microarrays. In order to identify changes in differentiation of the epidermis, we analyzed skin from E17.5 mice, when differentiation of the epidermis was more advanced.
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| Contributor(s) |
Lee B, Dai X |
| Citation(s) |
24735878 |
| Submission date |
Feb 17, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Xing Dai |
| E-mail(s) |
xdai@uci.edu
|
| Organization name |
UC Irvine
|
| Street address |
Med Sci I D288
|
| City |
Irvine |
| State/province |
Ca |
| ZIP/Postal code |
92692 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE55075 |
Ovol as gatekeepers of epithelial adhesion and differentiation |
|
| Relations |
| BioProject |
PRJNA238466 |
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