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Status |
Public on Nov 30, 2006 |
Title |
Effects of DKK1 on gene expression on melanocytes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Dickkopf 1 (DKK1), which is expressed at high mRNA levels by fibroblasts in the dermis of human skin on the palms and soles, inhibits the function and proliferation of melanocytes in the epidermis of those areas via the suppression of beta-catenin and microphthalmia-associated transcriptor factor (MITF). In this study, we investigated the effects of DKK1 on melanocyte gene expression profiles and on Wnt signaling pathways using DNA microarray technology. Keywords: compound treatment design
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Overall design |
Paired cDNA samples, labeled by cyanine 3- and cyanine 5-dUTP incorporation (Qiagen, Valencia, CA) during reverse transcription (Qiagen), were hybridized simultaneously with one oligo-DNA chip (HS-Operon V2vB2.2p13) as per NCI in-house protocol (available at http://mach1.nci.nih.gov/). Two fluorescent intensities of the oligo-DNA chip were scanned using a microarray scanner (GenePix 4000A, Axon Instruments, Inc., Molecular Device Corp., Sunnyvale, CA).
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Citation missing |
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Submission date |
Aug 14, 2006 |
Last update date |
Jan 17, 2013 |
Contact name |
Vincent Hearing |
E-mail(s) |
hearingv@nih.gov
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Organization name |
National Institutes of Health
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Department |
National Cancer Institute
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Lab |
Laboratory of Cell Biology
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Street address |
Bldg 37 Rm 2132
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City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
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Samples (1) |
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Relations |
BioProject |
PRJNA96163 |