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Series GSE55162 Query DataSets for GSE55162
Status Public on Feb 20, 2014
Title Age-related changes in the cellular composition and epithelial organization of the mouse trachea
Organism Mus musculus
Experiment type Expression profiling by array
Summary We report here senescent changes in the structure and organization of the mucociliary pseudostratified epithelium of the mouse trachea and the main stem bronchi. We confirm previous reports of the graduate appearance of age-related, gland-like structures (ARGLS) in the submucosa, espeically in the intercartilage regions and carina. Immunohistochemistry shows these structures contain ciliated and secretory cells and Krt5+ basal cells, but not the myoepithelial cells or ciliated ducts typical of normal submucosal glands. Data suggests they arise de novo by budding from teh surface epithelium rather than by delayted growth of small or cryptic submucosal glands. In old mice the surface epithelium contains fewer cells per unit length than in young mice and the proportion of Krt5+, p63+ basal cells is reduced in both males and females. However, there appears to be no significant difference in the ability of basal stem cells isolated from individual young and old mice to form clonal tracheospheres in culture or in the ability of the pithelium to repair after damage by inhaled sulfur dioxide. Gene expression analysis by Affymetrix microarray and quantitative PCR, as well as immunohistochemistry and flow sorting studies, are consistent with low-grade chronic inflammation in the tracheas of old versus young mice. The significance of these changes for ARGL formation are not clear since several treatments that induce acute inflammation in young mice did not result in budding of the surface epithelium.
Overall design Total RNA from distal tracheas and carinas of four young (2 month) and four older (14 month) C57Bl/6 female mice was extracted using QIAshredder and RNeasy Micro Kits (QIAGEN). The quality was checked with a 2100 Bioanalyzer (Agilent Technologies). Total RNA was processed using Ambion MessageAmpTM Premier by the Duke Microarray Facility. Standard Affymetrix protocols and Affymetrix GeneChip® Mouse Genome 430 2.0 Array chips were used to generate .cel files.
Contributor(s) Hogan BL, Wansleeben C
Citation(s) 24675804
Submission date Feb 19, 2014
Last update date Feb 11, 2019
Contact name Brigid Hogan
Phone 919-684-8085
Organization name Duke University Medical Center
Department Cell Biology
Lab Hogan Lab
Street address 388 Nanaline Duke Bldg PO Box 3709
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM1330619 Old1_Mouse430
GSM1330620 Old2_Mouse430
GSM1330621 Old5_Mouse430
BioProject PRJNA238657

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Supplementary file Size Download File type/resource
GSE55162_RAW.tar 30.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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