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Series GSE5518 Query DataSets for GSE5518
Status Public on Aug 18, 2006
Title Differential gene expression of Appresorium vs Vegetative mycelium of Magnaporthe using oligoarray
Organism Pyricularia grisea
Experiment type Expression profiling by array
Expression profiling by SAGE
Summary Rice blast, caused by the fungal pathogen Magnaporthe grisea, is a devastating disease causing tremendous yield loss in rice production. The public availability of the complete genome sequence of M. grisea provides ample opportunities to understand the molecular mechanism of its pathogenesis on rice plants at the transcriptome level. To identify all
the expressed genes encoded in the fungal genome, we have analyzed the mycelium and appressorium transcriptomes using MPSS, RL-SAGE and oligoarray methods.
Keywords: RL-SAGE, oligoarray
Overall design The M. grisea oliogoarray chip containing 60-mer oligos representing a total of 21,885 probes was obtained from Agilent (G4137A; Wilmington, Delaware). Of the 21,885 probes, 13,666 are from the annotated genes of M. grisea and 7,144 probes are from the rice ESTs ( The remainder 1,075 probes include quality controls, positive controls and negative controls.The oligoarray hybridization experiments included six technical replicates, of which three were dye-reversal.
Contributor(s) Wang G, Raghupathy MB, Gowda M, Reddychennavari V
Citation(s) 17156450
Submission date Aug 15, 2006
Last update date Jul 29, 2013
Contact name Mohan B Raghupathy
Phone 614 292 9280
Fax 614 292 4455
Organization name The Ohio State University
Department Department of Plant Pathology
Lab Plant disease resistance and fucntional genomics
Street address 201 Kottman Hall, 2021 Coffey Rd
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (2)
GPL4080 OSUGW_MG_21K_vA.7.1.1
GPL4085 SAGE:21:NlaIII:Magnaporthe grisea
Samples (2)
GSM126989 M. grisea strain (70-15)_total RNA from Vegetative mycelium and Appressorium
GSM127012 RL-SAGE_MGGW_Vegetative mycelium_Gowda et al., 2004
BioProject PRJNA96169

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Supplementary file Size Download File type/resource
GSE5518_RAW.tar 7.1 Mb (http)(custom) TAR (of TXT)

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