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Series GSE55361 Query DataSets for GSE55361
Status Public on Mar 11, 2015
Title Systematic Mapping of ADAR1 Binding Reveals its Regulatory Roles in Multiple RNA Processing Pathways [CLIP-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary ADARs are the primary factors underlying A-to-I editing in metazoans. We conducted the first global study of ADAR1-RNA interaction in human cells using CLIP-Seq. In contrast to the expected predominant binding of ADAR1 to Alu repeats, thousands of CLIP sites were located in non-Alu regions. This unexpectedly frequent non-Alu binding enabled discovery of transcriptome-wide functional and biophysical targets of ADAR1 in the regulation of mRNA processing including alternative 3' UTR usage and alternative splicing. In addition, a global analysis of ADAR1 binding to non-Alu regions also revealed its primary interaction with microRNA (miRNA) transcripts in the nucleus, which subsequently affected expression levels of mature miRNAs. A complex global picture was revealed regarding the dependence of this function on the double-stranded RNA binding domains or deaminase activity. Our study unfolded a broad landscape of the diverse functional roles of ADAR1.
 
Overall design To charaterize ADAR1 binding profiles in U87 cells, we performed CLIP-seq using two different ADAR1 monoclonal antibodies.
 
Contributor(s) Xiao X, Bahn J
Citation(s) 25751603
Submission date Feb 26, 2014
Last update date May 15, 2019
Contact name Jaegyoon Ahn
Organization name UCLA
Department IBP
Lab Xiao lab
Street address 611 Charles E. Young Drive
City LA
State/province CA
ZIP/Postal code 90095-1570
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (2)
GSM1334722 Adar1ClipAB1
GSM1334723 Adar1ClipAB2
This SubSeries is part of SuperSeries:
GSE55363 Systematic Mapping of ADAR1 Binding Reveals its Regulatory Roles in Multiple RNA Processing Pathways
Relations
BioProject PRJNA239413
SRA SRP038963

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Supplementary file Size Download File type/resource
GSE55361_ADAR1_clip_clusters.bed.gz 298.7 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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