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Status |
Public on Mar 11, 2015 |
Title |
Systematic Mapping of ADAR1 Binding Reveals its Regulatory Roles in Multiple RNA Processing Pathways [CLIP-seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
ADARs are the primary factors underlying A-to-I editing in metazoans. We conducted the first global study of ADAR1-RNA interaction in human cells using CLIP-Seq. In contrast to the expected predominant binding of ADAR1 to Alu repeats, thousands of CLIP sites were located in non-Alu regions. This unexpectedly frequent non-Alu binding enabled discovery of transcriptome-wide functional and biophysical targets of ADAR1 in the regulation of mRNA processing including alternative 3' UTR usage and alternative splicing. In addition, a global analysis of ADAR1 binding to non-Alu regions also revealed its primary interaction with microRNA (miRNA) transcripts in the nucleus, which subsequently affected expression levels of mature miRNAs. A complex global picture was revealed regarding the dependence of this function on the double-stranded RNA binding domains or deaminase activity. Our study unfolded a broad landscape of the diverse functional roles of ADAR1.
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Overall design |
To charaterize ADAR1 binding profiles in U87 cells, we performed CLIP-seq using two different ADAR1 monoclonal antibodies.
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Contributor(s) |
Xiao X, Bahn J |
Citation(s) |
25751603 |
Submission date |
Feb 26, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Jaegyoon Ahn |
Organization name |
UCLA
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Department |
IBP
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Lab |
Xiao lab
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Street address |
611 Charles E. Young Drive
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City |
LA |
State/province |
CA |
ZIP/Postal code |
90095-1570 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
GSE55363 |
Systematic Mapping of ADAR1 Binding Reveals its Regulatory Roles in Multiple RNA Processing Pathways |
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Relations |
BioProject |
PRJNA239413 |
SRA |
SRP038963 |