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| Status |
Public on Sep 26, 2015 |
| Title |
TG-interacting factor1 (Tgif1) maintains the identity of mouse ES cells by counterbalancing the expression of core pluripotency factors. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
TG-interacting factor1 (Tgif1) is well-known as a transcriptional repressor in transforming growth factor beta (TGFβ) signaling pathway. Target mapping of ES core factors in mouse embryonic stem (ES) cells revealed that Tgif1 is occupied by Oct4 and Nanog. Moreover, recent interactome study of mouse gene regulatory regions showed a preferential regulation of Tgif1 by mouse ES cell specific enhancers. However, the detailed role and mode of actions of Tgif1 in stem cell maintenance and development remains elusive. We show that Tgif1 is indispensable for self-renewal and pluripotency of mouse embryonic stem (ES) cells. Aberrant expression of Tgif1 promotes differentiation of ES cells even in the presence of LIF in part by deregulation of pluripotency factors. Intriguingly, we find that Tgif1 level is a critical factor to determine specific lineage commitment in a dosage-dependent manner. We further show that Tgif1 interacts with ES cell core factors and co-localizes at their binding sites, which eventually restricts expression of ES cell core factors including Oct4, Sox2, and Nanog. Taken together, we provide new insights into the roles of Tgif1 in maintenance as well as differentiation of ES cells.
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| Overall design |
We investigated the roles of Tgif1 in mouse embryonic stem (ES) cells by knocking down or overexpressing Tgif1 or Tgif2. Total RNAs were extracted from the ES cells upon KD and OE of Tgif1 or Tgif2, and then hybridized on Affymetrix Mouse GeneChip 430A 2.0. We monitored global gene expression changes upon perturbation of Tgif1 or Tgif2.
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| Contributor(s) |
Kim J |
| Citation(s) |
26411691 |
| Submission date |
Feb 26, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Bum-Kyu Lee |
| E-mail(s) |
bklee92@gmail.com
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| Phone |
512-232-8046
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| Organization name |
University of Texas at Austin
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| Street address |
2506 Speedway Stop A5000
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE55437 |
TG-interacting factor1 (Tgif1) maintains the identity of mouse ES cells by counterbalancing the expression of core pluripotency factors and ES cell core factors |
|
| Relations |
| BioProject |
PRJNA239580 |
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