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Series GSE55424 Query DataSets for GSE55424
Status Public on May 14, 2015
Title Regeneration occurs in the CNS of CAST/Ei mice as a consequence of enhanced Activin signaling
Organism Mus musculus
Experiment type Expression profiling by array
Summary We screened nine genetically diverse inbred mouse strains for differences in axonal growth of adult dorsal root ganglion (DRG) neurons on CNS myelin. Naïve DRG neurite outgrowth on myelin was very limited, but preconditioning the neurons by a prior sciatic nerve crush increased axonal growth substantially across all strains, with by far the greatest change in neurons from CAST/Ei mice. Three independent in vivo CNS injury models revealed greater capacity for CNS axonal regeneration in CAST/Ei than C57BL/6 mice. Full-genome expression profiling of naïve and pre-conditioned DRGs across all strains revealed Activin-βA (Inhba) as the transcript whose expression most closely correlated with axonal growth on myelin. In vitro and in vivo gain- and loss-of-function experiments confirmed that Activin promotes axonal growth in the CNS. Substantial regeneration is possible, therefore, in the injured mammalian CNS when Activin signaling is intrinsically high, as in CAST/Ei or when extrinsically modulated in other strains.
Overall design 9 strains, 4 replicates per strain, 2 conditions (naïve and axotomy) = 72 samples. 2 samples were excluded because technical outliers (AJ_AX5D_1 and AJ_NAIVE_4 excluded from the normalized data but included in the raw data)
Contributor(s) Omura T, Omura K, Riva P, Tedeschi A, Rojas L, Martin J, Huebner EA, Painter M, Latremoliere A, Yin Y, Barrett L, Singh B, Lee S, Crisman T, Gao F, Li S, Geschwind DH, Coppola G, He Z, Carmichael ST, Benowitz LI, Costigan M, Woolf CJ
Citation(s) 26004914
Submission date Feb 27, 2014
Last update date Jun 14, 2018
Contact name Giovanni Coppola
Phone 310-794-4172
Organization name UCLA
Department Psychiatry and Neurology
Lab Neurogenetics
Street address 1524 Gonda, 695 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (70)
GSM1336312 C3H_AX5D_3
GSM1336313 C57_NAIVE_3
GSM1336314 NZO_AX5D_1
BioProject PRJNA239562

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55424_RAW.tar 3.1 Mb (http)(custom) TAR
GSE55424_non-normalized.txt.gz 10.8 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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