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Series GSE5555 Query DataSets for GSE5555
Status Public on Jun 13, 2008
Title Hookworm-Induced Persistent Changes to the Immunological Environment of the Lung
Organism Mus musculus
Experiment type Expression profiling by array
Summary Infection with Nippostrongylus brasiliensis results in persistent changes to the lung environment. Cytokine profiling reveals a sustained increase in both Th1 and Th2 transcripts. Cellular populations of macrophages display an alternative phenotype, with upregulation of YM1, Arg1, Mrc1 as well as Class II MHC. These alternatively activated alveolar macrophages (AAAMs) also increase drastically in number. Subsequent challenge with house dust mite (HDM) Dermatophagoides pteronyssinus shows a reduced allergic phenotype, with decreased fold changes in effector cell cytokines of both the Th1 and Th2 variety indicative of the new regulatory environment established in the lung by helminth infection. Histological examination of the lung environment reveals a significant decrease in eosinophila and reduced mucous production by bronchial epithelial cells.
Keywords: Timecourse, Infection, Allergy Model
Overall design BALB/c mice were challenged with 500 infectious L3 stage Nippostrongylus brasiliensis larvae subcutaneously. Mice were allowed to resolve their infection, then were sensitized twice with 75 allergy units (AU) of HDM bound to 1mg of alum intraperitoneally. Two challenge doses of 50AU HDM in PBS were given intranasally one day apart, then lungs were harvested at 6, 24 and 72 hours post challenge. Mice were analyzed by histology, immunohistochemistry, real time RT-PCR and affymetrix gene array analysis using full genome chips.
Contributor(s) Reece JJ, Siracusa MC, Brayton CF, Southard TL, Urban JF, Scott AL
Citation(s) 18505812
Submission date Aug 17, 2006
Last update date Feb 11, 2019
Contact name Joshua J Reece
Organization name Johns Hopkins University BSPH
Department MMI
Lab Lab of Alan Scott
Street address 615 N. Wolfe Street
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (42)
GSM129094 CON_rep01
GSM129095 CON_rep02
GSM129096 CON_rep03
BioProject PRJNA96055

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Supplementary file Size Download File type/resource
GSE5555_RAW.tar 248.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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