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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 12, 2014 |
| Title |
Hippocampal neurons, Illumina experiments |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Ongoing neuronal activity during development and plasticity acts to refine synaptic connections and contributes to the induction of plasticity and ultimately long term memory storage. Activity-dependent post-transcriptional control of mRNAs occurs through transport to axonal and dendritic compartments, local translation, and mRNA stability. We have identified a mechanism that contributes to activity-dependent regulation of mRNA stability during synaptic plasticity. In this study we demonstrate rapid, post-transtriptional control over process-enriched mRNAs by neuronal activity. Systematic analysis of the 3'-UTRs of destablized transcripts, identifies enrichment in sequence motifs corresponding to miRNA binding sites. The miRNAs that were identified, miR-326-3p/miR-330-5p, miR-485-5p, miR-666-3p, and miR-761 are predicted to regulate networks of genes important in plasticity and development. We find that these miRNAs are developmentally regulated in the hippocampus, many increasing by postnatal day 14. We further show that miR-485-5p controls NGF-induced neurite outgrowth in PC12 cells, tau expression, and axonal development in hippocampal neurons. miRNAs can function at the synapse to rapidly control and affect short- and long-term changes at the synapse. These processes likely occur during refinement of synaptic connections and contribute to the induction of plasticity and learning and memory.
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| Overall design |
12 hippocampal cell culture samples analysed, 3 coverslips pooled per sample. Treatments are as follows:
Block: an inhibitor cocktail containing 50 µM D-APV, 40 µM CNQX, and 100 nM TTX for 3 hrs
Activity: 50 µM bicuculline (BiC)/500 µM 4-Aminopyridine
ActD: 25 µM actinomycin D
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| Contributor(s) |
Lee PR, Cohen JE, Fields RD |
| Citation(s) |
25135970 |
| Submission date |
Mar 11, 2014 |
| Last update date |
Sep 11, 2014 |
| Contact name |
Philip Lee |
| Organization name |
NIH
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| Department |
NICHD
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| Street address |
35 Lincoln Drive
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| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL6101 |
Illumina ratRef-12 v1.0 expression beadchip |
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| Samples (12)
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| GSM1345988 |
5min: BiC/4AP + ActD, Biological Replicate 3 |
| GSM1345989 |
30min: Block, Biological Replicate 3 |
| GSM1345990 |
30min: Block + ActD, Biological Replicate 3 |
| GSM1345991 |
30min: BiC/4AP, Biological Replicate 3 |
| GSM1345992 |
30min: BiC/4AP + ActD, Biological Replicate 3 |
| GSM1345993 |
60min: Block, Biological Replicate 3 |
| GSM1345994 |
60min: Block + ActD, Biological Replicate 3 |
| GSM1345995 |
60min: BiC/4AP, Biological Replicate 3 |
| GSM1345996 |
60min: BiC/4AP + ActD, Biological Replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE55781 |
Systematic identification of 3'UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons |
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| Relations |
| BioProject |
PRJNA241004 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55780_RAW.tar |
2.8 Mb |
(http)(custom) |
TAR |
| GSE55780_non-normalized.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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