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| Status |
Public on Jan 31, 2007 |
| Title |
Expression data from egy wildtype and mutant embryo comparison |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
U4/U6 di-snRNPs were disrupted and singular U4 and U6 snRNPs accumulated in egy mutant embryos, establishing the recycling function of p110 in vivo. Based on microarray analyses, a subset of spliceosome components and splicing-related factors was coordinately upregulated in the egy mutant. This revealed an extensive network of coregulated components of the spliceosome cycle, compensating – albeit inefficiently – for the recycling defect. In contrast, another set of genes, many of them eye- and pancreas-specific, was downregulated in the egy mutant embryos.
Keywords: mut / wt comparison
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| Overall design |
Zebrafish earl grey (egy) mutant embryos carry an autosomal recessive defect in the p110-orthologous gene which leads to microcephaly, microphthalmia, underdevelopment of the pharyngeal arches, and thymus hypoplasia by day 8 of development. To characterize the defect on the transcriptional level, egy whole embryos (n>100) were collected and morphologically separated into pools of mutant (mut) and wildtype (wt) sibling embryos. Pools of embryos were collected at 2 time points (3 and 4 days post fertilization) with 2 and 3 biological replicates, resp.. After RNA extraction, labelled cRNA was hybridized onto Affymetrix microarrays.
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| Contributor(s) |
Weber GJ, Trede N, Zhou Y, Zon LI |
| Citation(s) |
17416673 |
| Submission date |
Aug 24, 2006 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Gerhard J Weber |
| E-mail(s) |
gweber@enders.tch.harvard.edu
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| Organization name |
Children's Hospital Boston, Harvard Medical School
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| Department |
Hematology / Oncology
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| Street address |
1 Blackfan Circle
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (10)
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| GSM130391 |
egy mut sibling embryos at d3, biological rep1 |
| GSM130392 |
egy wt sibling embryos at d3, biological rep1 |
| GSM130393 |
egy mut sibling embryos at d3, biological rep2 |
| GSM130394 |
egy wt sibling embryos at d3, biological rep2 |
| GSM130395 |
egy mut sibling embryos at d5, biological rep1 |
| GSM130396 |
egy wt sibling embryos at d5, biological rep1 |
| GSM130397 |
egy mut sibling embryos at d5, biological rep2 |
| GSM130398 |
egy wt sibling embryos at d5, biological rep2 |
| GSM130399 |
egy mut sibling embryos at d5, biological rep3 |
| GSM130400 |
egy wt sibling embryos at d5, biological rep3 |
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| Relations |
| BioProject |
PRJNA95455 |
| Supplementary data files not provided |
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