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Series GSE5589 Query DataSets for GSE5589
Status Public on Aug 25, 2006
Title IL-10 or IL-6 stimulation of IL-6-/- and IL-10-/- murine bone marrow derived macrophages in the presence of LPS
Organism Mus musculus
Experiment type Expression profiling by array
Summary IL-10 or IL-6 stimulation of control 129xC57BL/6 murine bone marrow derived macrophages in the presence of LPS.
We used microarrays to detail the global programme of gene expression changes in response to IL-6 or IL-10 stimulation in the presence of lipopolysaccharide. BMDMs were isolated from control, IL-6-/-, and IL-10-/- mice on a 129XBL/6 mixed background mice and differentiated in the presence of CSF-1 for 6-7 days. Cells were scraped and plated in 6 well plates at 2x10e6/well. Cells were washed with complete DMEM and rested for 1-2 hr before stimulation with combinations of IL-10 (10 ng/ml), IL-6 (2 ng/ml) or LPS (100 ng/ml) for 45 min or 180 mins. Complete biological replicates were performed.
Keywords: time course
 
Overall design Data sets from wild-type, IL-10-/- and IL-6-/- BMDMs treated with IL-6 or IL-10 in the presence of LPS over time
 
Contributor(s) Murray PJ, Chen JD
Citation(s) 17114459
Submission date Aug 24, 2006
Last update date Feb 18, 2018
Contact name Peter J Murray
Organization name St. Jude Children's Research Hospital
Department Infectious Diseases
Street address 332 Nth Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platforms (1)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
Samples (34)
GSM130439 Murine bone marrow-derived macrophages at T0, biol. rep1
GSM130440 Murine bone marrow-derived macrophages at T0, biol. rep2
GSM130441 Murine bone marrow-derived macrophages at T0, biol. rep3
Relations
BioProject PRJNA95461

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