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| Status |
Public on Sep 24, 2006 |
| Title |
Profiling of zinc altered gene expression in human prostate normal (HPR-1) and malignant (PC-3) cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To gain a better understanding of the zinc effect on prostate cells, gene expression profiling analyses were conducted using HPR-1 and PC-3 cells treated with or without zinc (1,500 ng/ml) after 24 hrs serum-depletion. Zinc effect on gene expression was evaluated at 1, 3, and 6 hrs of zinc treatment compared to the control, using human genome survey microarray chips. A total of 6,110 identified genes exhibited three expression patterns in response to zinc a threshold of ±2.5 folds: suppressed (3,534 in PC-3; 1,953 in HPR-1), increased (571 in PC-3; 872 in HPR-1), and otherwise altered (16 in PC-3; 13 in HPR-1).
Keywords: Zinc effect, time course, cell type comparison
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| Overall design |
Zinc effect on gene expression of HPR-1 and PC-3 cells was evaluated with a time-course study using human genomewise microarray chips (Applied Biosystems v 2.0). For each chip, 2µg total RNA from each sample was used. A series of samples from each cell line was prepared as follows: control, 1 hr, 3 hrs, and 6 hrs zinc exposure. Synthesis of cRNA and subsequent hybridization was completed by the Core Facility at UMBI according to the ABI standard protocol. The ABI human genome survey microarray chip contained 32,878 probes for the interrogation of 29,098 known genes
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| Contributor(s) |
Lin S, Wei H, Hong Y, Feng P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 24, 2006 |
| Last update date |
Jan 27, 2016 |
| Contact name |
Shufei Lin |
| E-mail(s) |
slin@umaryland.edu
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| Phone |
410-706-7340
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| Fax |
410-706-7340
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| Organization name |
University of Maryland at Baltimore
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| Department |
Biomedical Sciences
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| Lab |
4D-08 Dental School
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| Street address |
666 W. Baltimore Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
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| Platforms (1) |
| GPL2986 |
ABI Human Genome Survey Microarray Version 2 |
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| Samples (8)
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| GSM129296 |
Profiling of altered gene expression induced by 1 hr of zinc in human prostate cells |
| GSM129421 |
Profiling of gene expression in human prostate cells |
| GSM129470 |
Profiling of altered gene expression induced by 3 hrs zinc in human prostate cells |
| GSM129471 |
Profiling of altered gene expression induced by 6 hrs of zinc in human prostate cells |
| GSM129472 |
Profiling of gene expression in human prostate cancer cells |
| GSM129473 |
Profiling of altered gene expression induced by 1 hr of zinc in human prostate cancer cells |
| GSM129474 |
Profiling of altered gene expression induced by 3 hr of zinc in human prostate cancer cells |
| GSM129475 |
Profiling of altered gene expression induced by 6 hr of zinc in human prostate cancer cells |
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| Relations |
| BioProject |
PRJNA96879 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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