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Series GSE55900 Query DataSets for GSE55900
Status Public on Mar 14, 2014
Title Low-density lipoprotein receptor-5 contributes to whole body metabolism by regulating fatty acid metabolism in the osteoblast
Organism Mus musculus
Experiment type Expression profiling by array
Summary Wnt signaling is critical for normal skeletal development as well as whole-body metabolic function. In this study, we described a previously unrecognized function for Wnt-Lrp5 signaling in the osteoblast that allows bone to acquire the resources necessary to fuel bone formation. Mice lacking the Lrp5 co-receptor specifically in osteoblasts and osteocytes exhibit the expected reductions in postnatal bone mass and also develop peripheral adiposity with corresponding reductions in energy expenditure. Conversely, mice expressing a high-bone mass mutant Lrp5 allele are leaner with reduced plasma triglyceride and free fatty acid levels. In this context, Wnt-initiated signals downstream of Lrp5, but not Lrp6, regulate the expression of key enzymes required for fatty acid β-oxidation. These results suggest that Wnt-Lrp5 signaling regulates basic cellular activities beyond those associated with fate-specification and differentiation in bone and that the skeleton influences global energy homeostasis via mechanisms independent of osteocalcin and glucose metabolism
Overall design Total RNA isolated from cultures of Lrp5-deficient osteoblasts differentiated for 7 days in vitro compared to control osteoblasts
Contributor(s) Riddle RC, Farber CR
Citation(s) 25802278
Submission date Mar 13, 2014
Last update date Jun 14, 2018
Contact name Charles R Farber
Organization name University of Virginia
Department Center for Public Health Genomics
Street address P.O. Box 800717
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1348210 Osteoblasts_Lrp5_Knockout_Day7_rep1
GSM1348211 Osteoblasts_Lrp5_Control_Day7_rep1
GSM1348212 Osteoblasts_Lrp5_Knockout_Day7_rep2
BioProject PRJNA241275

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55900_RAW.tar 3.1 Mb (http)(custom) TAR
GSE55900_non_normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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