|
| Status |
Public on Mar 18, 2014 |
| Title |
Gene Expression Profiling of Prostate Benign and Malignant Tissue |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We profiled genome-wide gene expression of human prostate benign and malignant tissue to identify potential biomarkers and immunotherapy targets.
We stratified malignant specimens according to their TMPRSS2:ERG gene fusion status.
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| Overall design |
Radical prostatectomy tissue samples were obtained from the Hershey Foundation Prostate Cancer Serum and Tumor Bank at our institution. Morphologic diagnosis was done by a pathologist. OCT blocks containing >30% of PCa tissue (with Gleason score of 6 or 7) were selected for RNA purification. A biopsy punch was used to select the PCa tissues from the OCT sample blocks. Benign or PCa tissues were homogenized using a TissueLyser (Qiagen) at 28 Hz for 5 min. Total RNA was isolated using Trizol reagent. RNA was quantified by NanoDrop ND-1000 spectrophotometer, and quality was evaluated with Agilent RNA 6000 NanoChip and the 2100 Bioanalyzer, with 28S/18S ratios and RIN determined by 2100 Expert software.
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| |
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| Contributor(s) |
Arredouani MS, Lu B, Sanda M |
| Citation(s) |
19737960 |
| Submission date |
Mar 17, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
M. Simo Arredouani |
| E-mail(s) |
simarred@gmail.com
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Surgery
|
| Street address |
330 Brookline Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA241405 |
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