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| Status |
Public on Mar 26, 2014 |
| Title |
Expression data from Adult Drosophila with controled microbiota |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The microbial population that live within the gut of animals influences their physiology. We used axenic and recolonized flies to identify genes whose expression is modulated by the presence of a bacterial flora in the gut.
We identified several up regulated genes, most of which are described as enriched in the midgut, and related either to immunity or to metabolism. This work also suggests that most microbiota regulated genes are Relish dependent.
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| Overall design |
We raised axenic Flies and either : (1) kept them germ free or (2) recolonized their environment with a set of 4 known commensal bacteria of lab-raised drosophila (Commensalibacter intestini, Lactobacillus plantarum, Lactobacillus brevis, Acetobacter pomorum). Flies were maintained in their respective (axenic or recolonized) environment from emergence to 7 days of age. Then females were collected, and total RNA extraction was performed on groups of 20 whole bodies.
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| Contributor(s) |
Combe BE, Defaye A, Bozonnet N, Puthier D, Royet J, Leulier F |
| Citation(s) |
24733183 |
| Submission date |
Mar 25, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
François Leulier |
| E-mail(s) |
francois.leulier@ens-lyon.fr
|
| Organization name |
Institut of Functional Genomics Lyon
|
| Street address |
46, allée d'Italie
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| City |
Lyon |
| ZIP/Postal code |
69364 |
| Country |
France |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| GSM1357125 |
Recolonized flies, biological replicate 1 |
| GSM1357126 |
Recolonized flies, biological replicate 2 |
| GSM1357127 |
Recolonized flies, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA242637 |
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