|
| Status |
Public on Jan 08, 2007 |
| Title |
Comparative transcriptome analysis of wild type and two knockout alleles of At-CAMTA |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Calcium has been shown to be an important signalling molecule in the transduction of abiotic stress signals in Arabidopsis thaliana. Alteration of the calcium signature through the use of inhibitors has been shown to result in changes in the expression of certain downstream abiotic stress-induced genes. However, the communication of this signal through specific calcium-sensitive intermediate molecules has remained poorly elucidated. Various candidate molecules exist and including calcinurin, calcium-dependent protein kinases and calmodulin.
Recently, a screen of an Arabidopsis thaliana cDNA expression library with calmodulin resulted in the isolation of the protein designated calmodulin-binding transcriptional activator or AtCAMTA that may function as one of these calcium-sensitive intermediate molecules. The Arabidopsis genome contains six CAMTA genes (AtCAMTA1-6), all of which are expressed in varying tissues throughout development. AtCAMTA1 was shown to function as a transcriptional activator through the expression of a chimeric protein consisting of the bacteria LexA protein fused to various segments of AtCAMTA expressed in yeast. In order to identify putative target genes of AtCAMTA1 in Arabidopsis we plan to analyse the transcriptome of AtCAMTA1 loss-of-function mutants. The gene expression pattern of two previously characterised alleles of AtCAMTA1 will be compared to wild type.
Experimenter name = Sarah Scrase-Field
Experimenter phone = 01865 275062
Experimenter fax = 01865 275074
Experimenter department = University of Oxford
Experimenter address = Department of Plants Sciences
Experimenter address = University of Oxford
Experimenter address = South Parks Road
Experimenter address = Oxford
Experimenter zip/postal_code = OX1 3RB
Experimenter country = UK
Keywords: genetic_modification_design
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| |
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| Overall design |
4 samples were used in this experiment
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| |
|
| Contributor(s) |
Scrase-Field S, Knight M, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 25, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (4)
|
| GSM131217 |
Scrase-Field_1-1_Col-0-wildtype_Rep1_ATH1 |
| GSM131218 |
Scrase-Field_1-2_camta1-2-Col-0_Rep1_ATH1 |
| GSM131219 |
Scrase-Field_1-3_Col-0-wildtype_Rep2_ATH1 |
| GSM131220 |
Scrase-Field_1-4_camta1-2-Col-0_Rep2_ATH1 |
|
| Relations |
| BioProject |
PRJNA96915 |
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