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| Status |
Public on Mar 27, 2014 |
| Title |
Dectin-1-mediated signaling leads to characteristic gene expressions in rat mast cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Rat mast cell line RBL-2H3 was analyzed to investigate the molecular mechanism of Dectin-1-mediated activation and responses of mast cells.
Dectin-1-mediated signaling stimulated characteristic gene expression, such as MCP-1, IL-4, IL-13, TNF-αand Nfkbiz.
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| Overall design |
RBL-2H3 cell line stably expressing Dectin-1 was established. Non-stimulated cells (control) compared to Dectin-1-stimulated (Curdlan) cells in order to comfirm Dectin-1-mediated signaling-inducible genes. Dectin-1-stimulated cells compared to Syk inhibitor R406-pretreated cells in order to examine the importance of Syk for Dectin-1-mediated gene up-regulations.
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| Contributor(s) |
Kimura Y, Chihara K, Honjoh C, Takeuchi K, Yamauchi S, Fujieda S, Sada K |
| Citation(s) |
25246527 |
| Submission date |
Mar 26, 2014 |
| Last update date |
Dec 04, 2014 |
| Contact name |
Yukihiro Kimura |
| E-mail(s) |
kimyuki@u-fukui.ac.jp
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| Organization name |
University of Fukui
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| Street address |
23-3 Matsuoka-Shimoaizuki
|
| City |
Eiheiji |
| State/province |
Fukui |
| ZIP/Postal code |
910-1193 |
| Country |
Japan |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA242739 |
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