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| Status |
Public on Mar 28, 2015 |
| Title |
Rat ventral prostate gland gene profile after androgen deprivation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Androgens are required for prostate development, growth and physiology, by activating the androgen receptor (AR) upon activation by testosterone and dihydrotestosterone (DHT), the AR undergoes conformational changes, dimerizes and translocates to the cell nucleus regulation important genes releted to cell survival. Understanding the mechanisms of androgen regulation in the prostate gland is important, because the prostate is affected by several different diseases, in particular prostate cancer (PCa). Several ways exist to treat prostate cancer and promote epithelial cell death. Treatments involving androgen manipulation include surgical castration (bilateral orchiectomy), antiandrogens (usually AR antagonists), or substances that inhibit androgen synthesis (5 alpha-reductase inhibitors, gonadotrophin-releasing hormone blockers). 17 beta-estradiol exerts anti-androgen effects by blocking the hypothalamic production of gonadotropin-releasing hormone and thereby inhibiting the production of testosterone by the testes , but also acts locally via interactions with either of the estrogen receptors found in the gland. It is known that the kinetics of apoptosis are different in the rat ventral prostate (VP) of castrated rats (Cas group) and in rats subjected to 17 beta-estradiol high dose (group E2) or their combination (group Cas+E2), with an evident additive effect in the latter situation (Garcia-Florez et al, 2005).
The microarray approach was done to figure out what genes are expressed and how the cells of ventral prostate gland responses when the androgen is not available comparing three diferent androgen deprivation methods (sirurgical castration, high dose of 17-beta estradiol and both treatment combined).
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| Overall design |
Forty-eight 21-day-old male Wistar rats were obtained from the Multidisciplinary Center for Biological Research (CEMIB), University of Campinas. The animals were kept under normal light conditions (12-h light:dark cycle) and received filtered tap water and Purina rodent chow ad libitum. On the 90th day after birth, the rats were divided in four groups (n=3) and assigned to different treatment groups. To cause androgen deprivation, we utilized three different procedures with different effects on epithelial cell apoptosis. Animals in the first group were castrated (Cas) by orchiectomy via scrotal incision under ketamine (150 mg/Kg body weight) and xylazin (10 mg/kg body weight) anesthesia. Animals in the second group received a 25 mg/Kg body weight dose of 17β-estradiol diluted in corn oil (E2 group). The third group received a combination of both treatments (Cas+E2 group) (combined orchiectomy and 17β-estradiol). In the control group (Ct; normal androgen and estrogen), the animals received only the vehicle. Three days after the treatments, the rats were killed by anesthetic overdose, and the ventral prostate was dissected out for the microarray and immunohistochemistry analyses.
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| Contributor(s) |
Rosa-Ribeiro R, Carvalho HF |
| Citation missing |
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| Submission date |
Mar 27, 2014 |
| Last update date |
Mar 28, 2015 |
| Contact name |
Rafaela da Rosa Ribeiro |
| E-mail(s) |
rafaela.darosa@hotmail.com
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| Organization name |
State University of Campinas
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| Department |
Structural and functional biology department
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| Street address |
Charles Dawin st, bl.N room 10
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| City |
Campinas |
| ZIP/Postal code |
13083-863 |
| Country |
Brazil |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1358418 |
Castrated animals_for three days_rep1 |
| GSM1358419 |
Castrated animals_for three days_rep2 |
| GSM1358420 |
Castrated animals_for three days_rep3 |
| GSM1358421 |
One high dose of 17beta-estradiol_rep1 |
| GSM1358422 |
One high dose of 17beta-estradiol_rep2 |
| GSM1358423 |
One high dose of 17beta-estradiol_rep3 |
| GSM1358424 |
Castrated animals plus one high dose of estradiol_rep1 |
| GSM1358425 |
Castrated animals plus one high dose of estradiol_rep2 |
| GSM1358426 |
Castrated animals plus one high dose of estradiol_rep3 |
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| Relations |
| BioProject |
PRJNA242866 |
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