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Series GSE56332 Query DataSets for GSE56332
Status Public on Mar 28, 2014
Title GATA1s induces hyperproliferation of eosinophil precursors in Down syndrome transient leukemia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transient leukemia (TL) is evident in 5-10% of all neonates with Down syndrome (DS) and associated with N-terminal truncating GATA1-mutations (GATA1s). Here we analyzed the effect of on gene expression upon ectopic expression of Gata1s or Gata1, while simultaneously knocking down endogenous GATA1, in wild-type CD34+-hematopoietic stem and progenitor cells during myeloid differentiation.
Ectopic expression of Gata1s, but not Gata1, in wild-type CD34+-hematopoietic stem and progenitor cells induced hyperproliferation of eosinophil promyelocytes in vitro. While GATA1s retained the function of GATA1 to induce eosinophil genes by occupying their promoter regions, GATA1s was impaired in its ability to repress oncogenic MYC and the pro-proliferative E2F transcription network.
 
Overall design We lentivirally transduced wild-type CD34+-hematopoietic stem and progenitor cells to ectopically express Gata1s or Gata1, while simultaneously knocking down endogenous GATA1, and cultured them in myeloid differentiation for 0, 4 and 14 days.
 
Contributor(s) Maroz A, Klusmann J
Citation(s) 24336126
Submission date Mar 28, 2014
Last update date Jan 23, 2019
Contact name Jan-Henning Klusmann
E-mail(s) jan-henning.klusmann@kgu.de
Organization name Goehte University Frankfurt
Department Pediatric Hematology and Oncology
Street address Theodor-Stern-Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (9)
GSM1359240 G1_1
GSM1359241 G1_2
GSM1359242 G1S_1
Relations
BioProject PRJNA242933

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56332_RAW.tar 19.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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