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Series GSE56365 Query DataSets for GSE56365
Status Public on Apr 01, 2014
Title Epidermal cells help coordinate leukocyte migration during inflammation through fatty acid-fueled matrix metalloproteinase production
Organism Danio rerio
Experiment type Expression profiling by array
Summary In addition to satisfying the metabolic demands of cells, mitochondrial metabolism helps regulate immune cell function. To date, such cell-intrinsic metabolic-immunologic cross-talk has only been described operating in cells of the immune system. Here we show that epidermal cells utilize fatty acid β-oxidation to fuel their contribution to the immune response during cutaneous inflammation. By live imaging metabolic and immunological processes within intact zebrafish embryos during cutaneous inflammation, we uncover a mechanism where elevated β-oxidation-fueled mitochondria-derived reactive oxygen species within epidermal cells helps guide matrix metalloproteinase-driven leukocyte recruitment. This mechanism requires the activity of a zebrafish homolog of the mammalian mitochondrial enzyme, Immunoresponsive gene 1. This study describes the first example of metabolic reprogramming operating within a non-immune cell type to help control its contribution to the immune response. Targeting of this metabolic-immunologic interface within keratinocytes may prove useful in treating inflammatory dermatoses.
In this study, Affymetrix Zebrafish Genome Arrays were used to identify zebrafish Irg1l (a homolog of mammalian IRG1) as a gene up-regulated in response to Salmonella infection.
Overall design The microarray analysis compared 4 day post fertilisation (dpf) dissected larval zebrafish trunks (approximately 50) that had been injected at 2 dpf with either: (i) PBS (as a negative control) or (ii) live Salmonella enterica serovar Typhimurium bacteria. The study was performed in triplicate.
Contributor(s) Print CG, Hall CJ, Crosier PS
Citation(s) 24852213
Submission date Mar 31, 2014
Last update date Jan 25, 2018
Contact name Cris Print
Organization name Auckland University
Street address Grafton Campus, Park Rd
City Auckland
ZIP/Postal code 1
Country New Zealand
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM1359950 control embryo: biological replicate 1
GSM1359951 infected embryo: biological replicate 1
GSM1359952 control embryo: biological replicate 2
BioProject PRJNA243056

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Supplementary file Size Download File type/resource
GSE56365_RAW.tar 11.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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