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| Status |
Public on Apr 01, 2014 |
| Title |
Epidermal cells help coordinate leukocyte migration during inflammation through fatty acid-fueled matrix metalloproteinase production |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
In addition to satisfying the metabolic demands of cells, mitochondrial metabolism helps regulate immune cell function. To date, such cell-intrinsic metabolic-immunologic cross-talk has only been described operating in cells of the immune system. Here we show that epidermal cells utilize fatty acid β-oxidation to fuel their contribution to the immune response during cutaneous inflammation. By live imaging metabolic and immunological processes within intact zebrafish embryos during cutaneous inflammation, we uncover a mechanism where elevated β-oxidation-fueled mitochondria-derived reactive oxygen species within epidermal cells helps guide matrix metalloproteinase-driven leukocyte recruitment. This mechanism requires the activity of a zebrafish homolog of the mammalian mitochondrial enzyme, Immunoresponsive gene 1. This study describes the first example of metabolic reprogramming operating within a non-immune cell type to help control its contribution to the immune response. Targeting of this metabolic-immunologic interface within keratinocytes may prove useful in treating inflammatory dermatoses.
In this study, Affymetrix Zebrafish Genome Arrays were used to identify zebrafish Irg1l (a homolog of mammalian IRG1) as a gene up-regulated in response to Salmonella infection.
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| Overall design |
The microarray analysis compared 4 day post fertilisation (dpf) dissected larval zebrafish trunks (approximately 50) that had been injected at 2 dpf with either: (i) PBS (as a negative control) or (ii) live Salmonella enterica serovar Typhimurium bacteria. The study was performed in triplicate.
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| Contributor(s) |
Print CG, Hall CJ, Crosier PS |
| Citation(s) |
24852213 |
| Submission date |
Mar 31, 2014 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Cris Print |
| E-mail(s) |
c.print@auckland.ac.nz
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| Organization name |
Auckland University
|
| Street address |
Grafton Campus, Park Rd
|
| City |
Auckland |
| ZIP/Postal code |
1 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (6)
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| GSM1359950 |
control embryo: biological replicate 1 |
| GSM1359951 |
infected embryo: biological replicate 1 |
| GSM1359952 |
control embryo: biological replicate 2 |
| GSM1359953 |
infected embryo: biological replicate 2 |
| GSM1359954 |
control embryo: biological replicate 3 |
| GSM1359955 |
infected embryo: biological replicate 3 |
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| Relations |
| BioProject |
PRJNA243056 |
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