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| Status |
Public on Apr 01, 2014 |
| Title |
Follicular B (FoB) cells purified from miR-17-92 transgenic mice, stimulated with LPS/IL-4 for 13.5 or 25.5 hours |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have generated a miR-17-92 transgenic allele (termed miR-17-92 Tg) whose expression can be turned on conditionally by Cre recombinase (Xiao et al, Nature Immunology, 9:405-14, 2008). The mice were crossed to CD19-Cre mice to turn on the transgene expression specifically in B cells.
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| Overall design |
Follicular B(FoB) cells were purified from CD19-Cre;miR-17~92 Tg/Tg mice (TG1, TG2, TG3) and miR-17~92 Tg/Tg mice (WT1, WT2, WT3) by MACS depletion of cells positive for CD5, CD43, and CD93 (also known as AA4.1). The purified B cells were stimulated with LPS/IL-4 for 13.5hr or 25.5hr.
The purity of follicular B cells was examined by flow cytometry and was greater than 95% for all samples. Total RNA was extracted using RNeasy kit (QIAGEN).
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| Contributor(s) |
Xiao C, Jin HY |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 31, 2014 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Steven Head |
| E-mail(s) |
shead@scripps.edu
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| Phone |
858 784-2263
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| Organization name |
The Scripps Research Institute
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| Street address |
10550 N Torrey Pines Rd
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE56379 |
Follicular B (FoB) cells purified from miR-17~92 TG and miR-17~92 tKO mice |
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| Relations |
| BioProject |
PRJNA243074 |
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