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Series GSE56421 Query DataSets for GSE56421
Status Public on Oct 02, 2014
Title Corneal Gene Expression Analysis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transplantation of amniotic membrane-expanded limbal epithelium (AMLE) in place of donor tissue grafts results in significantly improved outcomes for patients suffering from severe limbal stem cell deficiency; however the reasons for such superior results are unclear. The purpose of this study was to identify transcriptional gene profiles specific to AMLE and donor central corneal epithelium (CE), which may contribute to the divergent clinical outcomes observed following transplant. Limbal fibroblasts which underlie the epithelium and secrete extracellular matrix proteins following injury/surgery were also profiled. Using cell culture, immunofluorescence, microarray gene expression profiling and qRT-PCR validation; this study aims to identify enriched biological processes and pathways which characterise AMLE and CE tissues. We hope the study outcomes will shed light onto the factors which contribute to provide the improved clinical outcomes associated with AMLE transplantation.
 
Overall design Gene expression profiling of three central corneal buttons, three amniotic membrane-expanded limbal epithelial cultures and three limbal fibroblast cultures
 
Contributor(s) Gallagher C, Clarke C, Aherne ST, Katikireddy KR, Doolan P, Lynch V, Murphy W, Bobart Hone A, Murphy C, Clynes M, Power W, O'Sullivan F
Citation(s) 25125605
Submission date Apr 01, 2014
Last update date Jul 26, 2018
Contact name colin clarke
E-mail(s) colin.clarke@dcu.ie
Organization name Dublin City University
Street address Dublin City University
City Dublin
ZIP/Postal code d9
Country Ireland
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (9)
GSM1361185 Amniotic membrane expanded limbal epithelium-cultured-1
GSM1361186 Amniotic membrane expanded limbal epithelium-cultured-2
GSM1361187 Amniotic membrane expanded limbal epithelium-cultured-3
Relations
BioProject PRJNA243279

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56421_RAW.tar 38.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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