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Series GSE56591 Query DataSets for GSE56591
Status Public on Apr 09, 2014
Title Expression data from human monocyte derived macrophages infected with adenovirus expressing HIV-1 Vpr
Organism Homo sapiens
Experiment type Expression profiling by array
Summary HIV-1 Vpr protein is a multifunctional protein which perturbs human transcriptome and interacts with a number of cellular proteins. In this study, we have attempted to explore the efffects of Vpr on human transcriptome and have identified several genes which are involved in innate immune respone and cell signaling pathways.
We used the microarray analysis to elucidate the differnetail expression pattern of differnet genes in human macrophages infected with HIV-1 Vpr. As result we found that HIV-1 Vpr protein leads to the induction of various interferon stimualted genes (ISGs) and chemokines in human macrophages.
Overall design Human monocytes-derived macrophages (MDMs) were isolated from peripheral blood mononuclear cells (PBMCs) from two healthy donors and were infected with recombinant adenoviruses either expressing HIV-1 Vpr or ZsGreen1 as a control. At 48 hours post-infection, RNA was isolated and subjected to microarray analysis.
Contributor(s) Zahoor MA, Xue G, Fukumoto K, Yoko A
Citation(s) 25170834
Submission date Apr 08, 2014
Last update date Mar 25, 2019
Contact name Muhammad Atif Zahoor
Organization name University Health Network
Street address 101 College St.
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (4)
GSM1365128 Vpr-Mac-Donor1
GSM1365129 Zs-Mac-Donor1 Control
GSM1365130 Vpr-Mac-Donor2
BioProject PRJNA244012

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56591_Fold_change_2.txt.gz 140.7 Kb (ftp)(http) TXT
GSE56591_RAW.tar 31.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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