|
| Status |
Public on Nov 13, 2014 |
| Title |
Differential Gene Expression in PARP13 Knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Poly(ADP-ribose) Polymerase-13 (PARP13/ZAP/ZC3HAV1) is an antiviral factor, active against specific RNA viruses such as MLV, SINV and HIV. During infection PARP13 binds viral RNA via its 4 CCCH-type zinc finger domains and targets it for degradation by recruiting cellular mRNA decay factors such as the exosome complex and XRN1. Here we show that PARP13 binds to and regulates cellular mRNA under physiological conditions. Knock-down of PARP13 via RNAi results in the misregulation of hundreds of transcripts with an enrichment in signal peptide containing proteins. Among the most upregulated transcripts is TRILR4
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| |
|
| Overall design |
2 replicates of Control siRNA knock-down vs PARP13 siRNA knock-down
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| |
|
| Contributor(s) |
Chang P |
| Citation(s) |
25382312 |
| Submission date |
Apr 09, 2014 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Paul Chang |
| E-mail(s) |
pchang2@mit.edu
|
| Phone |
6173244057
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Chang
|
| Street address |
77 Massachusetts Ave
|
| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02139-4301 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (2) |
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| Relations |
| BioProject |
PRJNA244258 |
