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Series GSE5668 Query DataSets for GSE5668
Status Public on Sep 05, 2006
Title Identification and characterization of the changed and stable transcripts during mouse oocyte maturation
Organism Mus musculus
Experiment type Expression profiling by array
Summary There is massive destruction of transcripts during maturation of mouse oocytes. The objective of this project was to identify and characterize the transcripts that are degraded versus those that are stable during the transcriptionally silent germinal vesicle (GV)-stage to metaphase II (MII)-stage transition using the microarray approach. A system for oocyte transcript amplification using both internal and 3’-poly(A) priming was utilized to minimize the impact of complex variations in transcript polyadenylation prevalent during this transition. Transcripts were identified and quantified using Affymetrix Mouse Genome 430 v2.0 GeneChip. The significantly changed and stable transcripts were analyzed using Ingenuity Pathways Analysis and GenMAPP/MAPPFinder to characterize the biological themes underlying global changes in oocyte transcripts during maturation. It was concluded that the destruction of transcripts during the GV to MII transition is a selective rather than promiscuous process in mouse oocytes. In general, transcripts involved in processes that are associated with meiotic arrest at the GV-stage and the progression of oocyte maturation, such as oxidative phosphorylation, energy production, and protein synthesis and metabolism, were dramatically degraded. In contrast, transcripts encoding participants in signaling pathways essential for maintaining the unique characteristics of the MII-arrested oocyte, such as those involved in protein kinase pathways, were the most prominent among those stables.
Keywords: Developmental stage (mature vs. immature) comparison
 
Overall design Comparison immature GV-stage oocyte (3 biological replicates) with mature MII-stage oocytes (3 biological replicates)
 
Contributor(s) Su Y, Sugiura K, Woo Y, Wigglesworth K, Kamadar S, Affourtit J, Eppig JJ
Citation(s) 17022963, 19834616
Submission date Aug 29, 2006
Last update date Mar 24, 2020
Contact name You-Qiang Su
E-mail(s) youqiang.su@jax.org
Organization name The Jackson Laboratory
Street address 600 Main Street
City Bar Harbor
State/province ME
ZIP/Postal code 04609
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM132592 Fully grown GV-stage oocytes
GSM132657 Fully grown GV-stage oocytes sample #2
GSM132658 Fully grown GV-stage oocyte sample #3
Relations
BioProject PRJNA96947

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Supplementary file Size Download File type/resource
GSE5668_RAW.tar 33.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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