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| Status |
Public on Jan 01, 2015 |
| Title |
Astrocyte transcriptome from Mecp2-deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mutations in the gene encoding the transcriptional modulator methyl-CpG binding protein 2 (MeCP2) are responsible for the neurodevelopmental disorder Rett syndrome that is one of the most frequent sources of intellectual disability in women. It has been shown that mutant astrocytes contribute to neuronal defects, probably as a result of aberrant secretion of soluble factor(s). Based on the hypothesis that this irregular secretion of soluble factor is the result of abnormal transcription of those factors, we have compared the gene expression profiles of wild type and mutant astrocytes from Mecp2 308/y mice by using Affymetrix mouse 2.0 microarrays.
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| Overall design |
P0 newborns from pregnant Mecp2 308/+ heterozygous mice were used for the preparation of dissociated cortical astrocyte cultures. Four wild-type and four mutant confluent astrocytes cultures were used for total RNA extraction with the Qiagen RNeasy kit (Qiagen, Courtaboeuf, France) as described by the manufacturer. The gene expression profiles of wild type and mutant astrocytes from Mecp2 308/y mice have been assayed by using Affymetrix mouse 2.0 microarrays.
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| Contributor(s) |
Delepine C, Bienvenu T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 10, 2014 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Florent Dumont |
| E-mail(s) |
florent.dumont@u-psud.fr
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| Organization name |
Université Paris-Saclay
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| Street address |
17 avenue des sciences
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| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| GSM1367115 |
Cortical astrocytes 7DIV, Mecp2 WT/y, biological rep 1 |
| GSM1367116 |
Cortical astrocytes 7DIV, Mecp2 308/y, biological rep 1 |
| GSM1367117 |
Cortical astrocytes 7DIV, Mecp2 308/y, biological rep 2 |
| GSM1367118 |
Cortical astrocytes 7DIV, Mecp2 WT/y, biological rep 4 |
| GSM1367119 |
Cortical astrocytes 7DIV, Mecp2 308/y, biological rep 3 |
| GSM1367120 |
Cortical astrocytes 7DIV, Mecp2 308/y, biological rep 4 |
| GSM1367121 |
Cortical astrocytes 7DIV, Mecp2 WT/y, biological rep 2 |
| GSM1367122 |
Cortical astrocytes 7DIV, Mecp2 WT/y, biological rep 3 |
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| Relations |
| BioProject |
PRJNA244332 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE56709_RAW.tar |
68.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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