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Series GSE56770 Query DataSets for GSE56770
Status Public on Jun 30, 2015
Title Expression data from rice roots treated with Gln
Platform organism Oryza sativa
Sample organism Oryza sativa Japonica Group
Experiment type Expression profiling by array
Summary We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by glutamine in rice roots.
Transcriptomic analysis of rice roots revealed that the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses was rapidly induced by glutamine within 30 minutes.
 
Overall design Total RNA from –N and Gln-treated roots (2.5 mM Gln, 30 min) was extracted using a phenol extraction protocol. RNA samples from two biological repeats were sent to the Affymetrix Gene Expression Service Lab at Academia Sinica, Taipei, Taiwan (http://ipmb.sinica.edu.tw/affy/) for target preparation, and hybridization to the GeneChip Rice Genome Array.
 
Contributor(s) Kan C, Chung T, Hsieh M
Citation(s) 26697351
Submission date Apr 14, 2014
Last update date Jan 25, 2018
Contact name Ming-Hsiun Hsieh
E-mail(s) ming@gate.sinica.edu.tw
Phone +886 2 2787 1168
Organization name Academia Sinica
Department Institute of Plant and Microbial Biology
Street address 128 Academia Road, Section 2
City Taipei
ZIP/Postal code 11529
Country Taiwan
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (4)
GSM1368814 root -N, biological rep 1
GSM1368815 root -N, biological rep 2
GSM1368816 root +gln, biological rep 1
Relations
BioProject PRJNA244515

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56770_RAW.tar 17.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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