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Status |
Public on Jun 30, 2015 |
Title |
Expression data from rice roots treated with Gln |
Platform organism |
Oryza sativa |
Sample organism |
Oryza sativa Japonica Group |
Experiment type |
Expression profiling by array
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Summary |
We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by glutamine in rice roots. Transcriptomic analysis of rice roots revealed that the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses was rapidly induced by glutamine within 30 minutes.
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Overall design |
Total RNA from –N and Gln-treated roots (2.5 mM Gln, 30 min) was extracted using a phenol extraction protocol. RNA samples from two biological repeats were sent to the Affymetrix Gene Expression Service Lab at Academia Sinica, Taipei, Taiwan (http://ipmb.sinica.edu.tw/affy/) for target preparation, and hybridization to the GeneChip Rice Genome Array.
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Contributor(s) |
Kan C, Chung T, Hsieh M |
Citation(s) |
26697351 |
Submission date |
Apr 14, 2014 |
Last update date |
Jan 25, 2018 |
Contact name |
Ming-Hsiun Hsieh |
E-mail(s) |
ming@gate.sinica.edu.tw
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Phone |
+886 2 2787 1168
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Organization name |
Academia Sinica
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Department |
Institute of Plant and Microbial Biology
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Street address |
128 Academia Road, Section 2
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City |
Taipei |
ZIP/Postal code |
11529 |
Country |
Taiwan |
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Platforms (1) |
GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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Samples (4)
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Relations |
BioProject |
PRJNA244515 |
Supplementary file |
Size |
Download |
File type/resource |
GSE56770_RAW.tar |
17.6 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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